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Team:KULeuven/6 September 2009
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Revision as of 14:17, 9 September 2009
Project progress
Progress of parts
[edit] Blue Light Receptor
- There was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. This was all done in a dark room to avoid activation of the promotor.
[edit] Vanillin Production
- Restriction digest overnight of sam5 (1,2) and sam8 (1,2)
- Growth of EF Ligation (woohoo!)
- Transferred 4 colonies from EF ligation to a new plate
- Put the 4 transferred colonies on liquid medium
A miniprep and restriction digest (ON) of sam5, sam8, ech and fcs was performed on Saturday. On Sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made.
