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Team:KULeuven/11 September 2009
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Latest revision as of 09:22, 10 September 2009
Project progress
Progress of parts
[edit] Blue Light Receptor
- The electroporated ligY did not grow. A new restriction digest on pcr product was performed with EcoRI and PstI.This was put on gel. There was a good signal so it was used to perform a ligation with vector pSB1A2.
- A liquid culture that was made from pSB3K3 a few days ago showed growth after all. This was miniprepped and restriction digested to check whether the vector is actually present.
- The liquid cultures with our blue light construct (lig) that stood overnight at 16°C was FACS-ed. Half of the cultures were exposed to blue light during the day and put under the FACS in the evening.
[edit] Vanillin Production
- Miniprep SAMS
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| SAMS1 1 | 118,2 | ||
| SAMS1 2 | 105,2 | ||
| SAMS1 3 | 95,7 | ||
| SAMS1 4 | 112,0 | ||
| SAMS2 1 | 67,8 | ||
| SAMS2 2 | 122,6 | ||
| SAMS2 3 | 90,0 | ||
| SAMS2 4 | 99,6 |
- Restriction SAMS + Promotor (
- Promotor restriction results in 2 parts of 800 bp and 2k bp
- EFT electroporation no result, try again on monday...
[edit] Vanillin Receptor
- Glycerolstock was made for virG and a sample was send for seqencing
- virA was ented
- new TOPOcloning was done for rpoA with a new protocol we recieved
