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Template:Team:KULeuven/8 September 2009/VanillinProduction
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JochemDeen (Talk | contribs) (New page: * Restriction digest on EF1, EF2, EF3 and EF4 ** Gel electrophorese of digestion, result: EF1 and EF3 appear to have an insert. The insert of EF4 is too short, beteen 1500 and 2000 bp, mig...) |
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* Restriction digest on EF1, EF2, EF3 and EF4 | * Restriction digest on EF1, EF2, EF3 and EF4 | ||
| - | ** Gel | + | ** Gel electrophoresis of digestion, result: EF1 and EF3 appear to have an insert. The insert of EF4 is too short, between 1500 and 2000 bp (might just be ech) and EF2 appears to have been cut by only 1 enzyme |
| - | ** Also restriction digest overnight results in an extra very weak intensity band at 600 and one between 1500 and 2000 | + | ** Also restriction digest overnight results in an extra very weak intensity band at 600 and one between 1500 and 2000 bp |
| - | *** This result has been seen before on just ech | + | *** This result has been seen before on just ech. It might be due to an extra restriction site on the ech gene. The two bands could result from this same band. The addition of both bands would then be the same as the insert. |
| - | * Electroporation of SAMS1 and | + | * Electroporation of SAMS1 and SAMS2 |
Revision as of 10:05, 10 September 2009
- Restriction digest on EF1, EF2, EF3 and EF4
- Gel electrophoresis of digestion, result: EF1 and EF3 appear to have an insert. The insert of EF4 is too short, between 1500 and 2000 bp (might just be ech) and EF2 appears to have been cut by only 1 enzyme
- Also restriction digest overnight results in an extra very weak intensity band at 600 and one between 1500 and 2000 bp
- This result has been seen before on just ech. It might be due to an extra restriction site on the ech gene. The two bands could result from this same band. The addition of both bands would then be the same as the insert.
- Electroporation of SAMS1 and SAMS2
