"
Team:KULeuven/Design/Blue Light Receptor
From 2009.igem.org
| Line 3: | Line 3: | ||
{{Team:KULeuven/Common2/EndHeader}} | {{Team:KULeuven/Common2/EndHeader}} | ||
| - | <html><div | + | <html><div id="centerall"></html> |
{{kulpart|BBa_K238013|DeepComponents}} | {{kulpart|BBa_K238013|DeepComponents}} | ||
{{kulpart|BBa_K238013}} | {{kulpart|BBa_K238013}} | ||
Revision as of 07:12, 14 September 2009
The purpose of the receptor is to enter the wanted vanillin concentration. The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. The light used to perform the test has a wavelength of 470nm.
We designed the part in such way that irradiation with a certain amount of blue light activates transcription of RIBOKEY-mRNA. To achieve this, we purified the promoter-region of the E. coli- strain MC4100. After mutating out possible restriction sites, the blr promoter part () was added to the registry.
