Template:Team:KULeuven/29 August 2009/VanillinProduction

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(Difference between revisions)

Latest revision as of 11:43, 14 September 2009

Split the restriction digest in 2 parts
- 1 Part was directly used for ligation using double the mass required(SAMS II and EF II)

Part	DNA ng	volume µl
sam5	100	0,8
sam8	126,4	2,7
ech	100	1
fcs	244	2,2

The 2nd part was first put on a gel electrophoresis and purified before it was used for ligation
Gel electrophoresis of restriction on sam5, sam8, fcs and ech
- sam5 and sam8 showed expected results
- fcs and ech had unexpected signals
  - fcs showed only 1 result on gel, expected two
  - ech showed 4 results in gel (all too short), expected one
- sam5 and sam8 were purified and ligated (SAMS I)

Part	DNA conc ng/µl	260/280
sam5	6,6	1,98
sam8	4,1	2,88

ech and fcs were cut again and put overnight (over the weekend...) in 16°C

@@ Line 1: / Line 1: @@
 * Split the restriction digest in 2 parts
-** 1 Part was directly used for ligation using double the mass required(Sams II and Ef II)
+** 1 Part was directly used for ligation using double the mass required(SAMS II and EF II)
 {| border ="1" align="center"
 !| Part || DNA ng || volume µl
 |- align="center"
-| Sam5||100 || 0,8
+| ''sam5''||100 || 0,8
 |- align="center"
-| Sam8||126,4 || 2,7
+| ''sam8''||126,4 || 2,7
 |- align="center"
-| Ech ||100 || 1
+| ''ech'' ||100 || 1
 |- align="center"
-| Fcs ||244 || 2,2
+| ''fcs'' ||244 || 2,2
 |-
 |}
-* The 2nd part was first put on gel electrophorese and purified before it was used for ligation
+* The 2nd part was first put on a gel electrophoresis and purified before it was used for ligation
-* Gel electrophorese of restriction on Sam, Sam8, fcs and ech
+* Gel electrophoresis of restriction on ''sam5, sam8, fcs and ech''
-** Sam5 and Sam8 showed expected results
+** ''sam5'' and ''sam8'' showed expected results
-** fcs and ech was unexpected
+** ''fcs'' and ''ech'' had unexpected signals
-***fcs showed only 1 result on gel, expected two
+***''fcs'' showed only 1 result on gel, expected two
-***ech showed 4 results in gel (all too short), expected one
+***''ech'' showed 4 results in gel (all too short), expected one
-** Sam5 and Sam8 were purified and ligated (Sams I)
+** ''sam5'' and ''sam8'' were purified and ligated (SAMS I)
 {| border ="1" align="center"
 !| Part || DNA conc ng/µl|| 260/280
 |- align="center"
-| Sam5||6,6 || 1,98
+| ''sam5''||6,6 || 1,98
 |- align="center"
-| Sam8||4,1 || 2,88
+| ''sam8''||4,1 || 2,88
 |-
 |}
-* Ech and Fcs were cut again and put overnight (overweekend...) in 16C
+* ''ech'' and ''fcs'' were cut again and put overnight (over the weekend...) in 16°C