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Template:Team:KULeuven/8 September 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
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* Restriction digest on EF1, EF2, EF3 and EF4 | * Restriction digest on EF1, EF2, EF3 and EF4 | ||
| - | ** Gel electrophoresis of digestion, result: EF1 and EF3 appear to have an insert. The insert of EF4 is too short, between 1500 and 2000 bp (might just be ech) and EF2 appears to have been cut by only 1 enzyme | + | ** Gel electrophoresis of digestion, result: EF1 and EF3 appear to have an insert. The insert of EF4 is too short, between 1500 and 2000 bp (might just be ''ech'') and EF2 appears to have been cut by only 1 enzyme |
| - | ** Also restriction digest overnight results in an extra very weak intensity band at 600 and one between 1500 and 2000 bp | + | ** Also, restriction digest overnight results in an extra very weak intensity band at 600 and one between 1500 and 2000 bp |
| - | *** This result has been seen before on just ech. It might be due to an extra restriction site on the ech gene. The two bands could result from this same band. The addition of both bands would then be the same as the insert. | + | *** This result has been seen before on just ''ech''. It might be due to an extra restriction site on the ''ech'' gene. The two bands could result from this same band. The addition of both bands would then be the same as the insert. |
* Electroporation of SAMS1 and SAMS2 | * Electroporation of SAMS1 and SAMS2 | ||
| - | * Glycerolstock for sam5, sam8, ech and fcs | + | * Glycerolstock for ''sam5, sam8, ech'' and ''fcs'' |
Latest revision as of 12:08, 14 September 2009
- Restriction digest on EF1, EF2, EF3 and EF4
- Gel electrophoresis of digestion, result: EF1 and EF3 appear to have an insert. The insert of EF4 is too short, between 1500 and 2000 bp (might just be ech) and EF2 appears to have been cut by only 1 enzyme
- Also, restriction digest overnight results in an extra very weak intensity band at 600 and one between 1500 and 2000 bp
- This result has been seen before on just ech. It might be due to an extra restriction site on the ech gene. The two bands could result from this same band. The addition of both bands would then be the same as the insert.
- Electroporation of SAMS1 and SAMS2
- Glycerolstock for sam5, sam8, ech and fcs
