"
Team:Chiba/Notebook/Calendar/13 September 2009
From 2009.igem.org
| Line 5: | Line 5: | ||
([[Team:Chiba/Notebook/Calendar/12_September_2009|12_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/14_September_2009|14_September_2009]]) | ([[Team:Chiba/Notebook/Calendar/12_September_2009|12_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/14_September_2009|14_September_2009]]) | ||
| - | + | == Check of Sender's AHL generation (3) == | |
| - | + | ||
*Main culture | *Main culture | ||
7:10- | 7:10- | ||
Revision as of 16:11, 14 September 2009
(12_September_2009 <|>14_September_2009)
Contents |
Check of Sender's AHL generation (3)
- Main culture
7:10-
- Measuring OD
12:20
OD600=1.98
- Wash
12:40
First, we dispensed culture solution about 12.5 mL to each 10 centrifuging tubes.
12:45
Next, we centrifuged samples 3 times(rpm=3000, 5 min, 20 degrees Celsius).
- Centrifugation
14:35
We centrifuged one of samples, and cultured the others at 37 degrees Celsius.
And then,
Test
18:05 Start
 (1)AHL 0 min. |
 (2)AHL 15 min. |
 (3) 30 min. |
 (4) 45 min. |
 (5) 60 min. |
 (6) 75 min. |
 (7) 90 min. |
 (8) 105 min. |
 (9) 120 min. |
 (10) 135 min. |
18:35
 (1)AHL 0 min. |
 (2)AHL 15 min. |
 (3) 30 min. |
 (4) 45 min. |
 (5) 60 min. |
 (6) 75 min. |
 (7) 90 min. |
 (8) 105 min. |
 (9) 120 min. |
 (10) 135 min. |
19:05
 (1)AHL 0 min. |
 (2)AHL 15 min. |
 (3) 30 min. |
 (4) 45 min. |
 (5) 60 min. |
 (6) 75 min. |
 (7) 90 min. |
 (8) 105 min. |
 (9) 120 min. |
 (10) 135 min. |
19:35
 (1)AHL 0 min. |
 (2)AHL 15 min. |
 (3) 30 min. |
 (4) 45 min. |
 (5) 60 min. |
 (6) 75 min. |
 (7) 90 min. |
 (8) 105 min. |
 (9) 120 min. |
 (10) 135 min. |
 N.C. |
20:05
 (1)AHL 0 min. |
 (2)AHL 15 min. |
 (3) 30 min. |
 (4) 45 min. |
 (5) 60 min. |
 (6) 75 min. |
 (7) 90 min. |
 (8) 105 min. |
 (9) 120 min. |
 (10) 135 min. |
 N.C. |
20:35
 (1)AHL 0 min. |
 (2)AHL 15 min. |
 (3) 30 min. |
 (4) 45 min. |
 (5) 60 min. |
 (6) 75 min. |
 (7) 90 min. |
 (8) 105 min. |
 (9) 120 min. |
 (10) 135 min. |
21:05
 (1)AHL 0 min. |
 (2)AHL 15 min. |
 (3) 30 min. |
 (4) 45 min. |
 (5) 60 min. |
 (6) 75 min. |
 (7) 90 min. |
 (8) 105 min. |
 (9) 120 min. |
 (10) 135 min. |
 N.C. |
21:35
 (1)AHL 0 min. |
 (2)AHL 15 min. |
 (3) 30 min. |
 (4) 45 min. |
 (5) 60 min. |
 (6) 75 min. |
 (7) 90 min. |
 (8) 105 min. |
 (9) 120 min. |
 (10) 135 min. |
 N.C. |
22:05
 (1)AHL 0 min. |
 (2)AHL 15 min. |
 (3) 30 min. |
 (4) 45 min. |
 (5) 60 min. |
 (6) 75 min. |
 (7) 90 min. |
 (8) 105 min. |
 (9) 120 min. |
 (10) 135 min. |
 N.C. |
