Team:Chiba/Notebook/Calendar/13 September 2009

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Revision as of 05:24, 15 September 2009

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Check of Sender's AHL generation (3)

Main culture

7:10-

Measuring OD

12:20

OD₆₀₀=1.98

Wash

12:40

First, we dispensed culture solution about 12.5 mL to each 10 centrifuging tubes.

12:45

Next, we centrifuged samples 3 times(rpm=3000, 5 min, 20 degrees Celsius).

Centrifugation

13:50

We added more 12.5 mL of liquid medium and 12.5 μL of 0.1 M IPTG to each tubes.

After that, we centrifuged one of samples, and cultured the others at 30 degrees Celsius.

And then, we picked 10 mL of supernatant solution and saved it.

-- We picked supernatant solution every 15 min.--

16:15

We prepared mixture and made solidified media using this.

mixture : 10 mL LB-agar solidified medium and 10 mL supernatant solution

17:20

We transferred colonies onto NC filters and cultured it at 37 degrees Celsius.

18:05

We put NC filters on plates which has been prepared a little while ago.

After that we started observation of GFP fluorescence.

Pictures are here.

Check of Sender's AHL generation

18:05 Start

(1)AHL 0 min.	(2)AHL 15 min.	(3) 30 min.	(4) 45 min.
(5) 60 min.	(6) 75 min.	(7) 90 min.	(8) 105 min.
(9) 120 min.	(10) 135 min.

18:35

(1)AHL 0 min.	(2)AHL 15 min.	(3) 30 min.	(4) 45 min.
(5) 60 min.	(6) 75 min.	(7) 90 min.	(8) 105 min.
(9) 120 min.	(10) 135 min.

19:05

(1)AHL 0 min.	(2)AHL 15 min.	(3) 30 min.	(4) 45 min.
(5) 60 min.	(6) 75 min.	(7) 90 min.	(8) 105 min.
(9) 120 min.	(10) 135 min.

19:35

(1)AHL 0 min.	(2)AHL 15 min.	(3) 30 min.	(4) 45 min.
(5) 60 min.	(6) 75 min.	(7) 90 min.	(8) 105 min.
(9) 120 min.	(10) 135 min.	N.C.

20:05

(1)AHL 0 min.	(2)AHL 15 min.	(3) 30 min.	(4) 45 min.
(5) 60 min.	(6) 75 min.	(7) 90 min.	(8) 105 min.
(9) 120 min.	(10) 135 min.	N.C.

20:35

(1)AHL 0 min.	(2)AHL 15 min.	(3) 30 min.	(4) 45 min.
(5) 60 min.	(6) 75 min.	(7) 90 min.	(8) 105 min.
(9) 120 min.	(10) 135 min.

21:05

(4)-(10) were became luminescent.

(1)AHL 0 min.	(2)AHL 15 min.	(3) 30 min.	(4) 45 min.
(5) 60 min.	(6) 75 min.	(7) 90 min.	(8) 105 min.
(9) 120 min.	(10) 135 min.	N.C.

21:35

(3) has became luminescent.

(1)AHL 0 min.	(2)AHL 15 min.	(3) 30 min.	(4) 45 min.
(5) 60 min.	(6) 75 min.	(7) 90 min.	(8) 105 min.
(9) 120 min.	(10) 135 min.	N.C.

22:05

(1)AHL 0 min.	(2)AHL 15 min.	(3) 30 min.	(4) 45 min.
(5) 60 min.	(6) 75 min.	(7) 90 min.	(8) 105 min.
(9) 120 min.	(10) 135 min.	N.C.

Revision as of 05:22, 15 September 2009 (view source) Kaoru Yamamoto (Talk \| contribs) ← Older edit		Revision as of 05:24, 15 September 2009 (view source) Kaoru Yamamoto (Talk \| contribs) (→Check of Sender's AHL generation (3)) Newer edit →
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-	Pictures are here.	+	'''Pictures are here.'''