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Team:Warsaw/Calendar-Main/15 April 2009
From 2009.igem.org
(Difference between revisions)
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<h3>PCR mgtc, hly</h3> | <h3>PCR mgtc, hly</h3> | ||
| - | <h4>Kamil | + | <h4>Kamil</h4> |
<br /> | <br /> | ||
<p>Tasks:</p> | <p>Tasks:</p> | ||
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<li>Amplification of mgtc and hly</li> | <li>Amplification of mgtc and hly</li> | ||
</ul> | </ul> | ||
| + | <br /> | ||
<p>Methods:</p> | <p>Methods:</p> | ||
<ul> | <ul> | ||
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<li>Electrophoretic separation on 1% agarose gel</li> | <li>Electrophoretic separation on 1% agarose gel</li> | ||
</ul> | </ul> | ||
| + | <br /> | ||
<p>Results:</p> | <p>Results:</p> | ||
<img src="https://static.igem.org/mediawiki/2009/1/1f/Obrazek.jpg"/> | <img src="https://static.igem.org/mediawiki/2009/1/1f/Obrazek.jpg"/> | ||
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</ol> | </ol> | ||
</var> | </var> | ||
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<p>Notes:</p> | <p>Notes:</p> | ||
<p>Inappropriate template DNA was used for mgtc (ganomic DNA from Yersinia instead of genomic DNA from Listeria).</p> | <p>Inappropriate template DNA was used for mgtc (ganomic DNA from Yersinia instead of genomic DNA from Listeria).</p> | ||
Revision as of 21:42, 9 June 2009
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PCR mgtc, hly
Kamil
Tasks:
- Amplification of mgtc and hly
Methods:
PCR mixture's composition:
2ul buffer*, 1ul MgCl2*, 0,5ul primers, 1,5ul dNTPs (10 mM), 01,ul polymerase**, solution was topped up with H2O to 20ul.2 repeats of every sample were made.
* buffer and Mg made by Grubs
** polymerase pfu turbo from first dilution made by Ziemniak
- PCR program:
- mgtc
90s 95°C, 2x(30s 95°C, 35s 48°C, 60s 72°C), 28x(30s 95°C, 35s 58°C, 60s 72°C), 600s 72°C, ~ 4°C- hly
90s 95°C, 2x(30s 95°C, 35s 42°C, 150s 72°C), 28x(30s 95°C, 35s 47°C, 150s 72°C), 600s 72°C, ~ 10°C- Electrophoretic separation on 1% agarose gel
Results:
Gel (from left)
- GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
- mgtc 1 repeat
- mgtc 2 repeat
- mgtc sample -
- hly 1 repeat
- hly 2 repeat
- hly sample -
Notes:
Inappropriate template DNA was used for mgtc (ganomic DNA from Yersinia instead of genomic DNA from Listeria).
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