Team:Nevada/Notebook

From 2009.igem.org

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(Gene 3-Cynnamaldehyde Reductase)
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== Gene 3-Cynnamaldehyde Reductase ==
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== June 8, 2009 to June 15, 2009 ==
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'''Janice/Leigh:''' Made LB agar plates and liquid LB with tetracycline.  Made tetracycline stock solution (5mg/ml).
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== June 16, 2009 to June 23, 2009 ==
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'''Janice/Leigh:''' Made LB liquid media and performed QIAGEN Plasmid Maxi Preps for 1) BBa_B0014 in pSB1AK3 (double terminator), 2) BBA_B0034 in pSB1A3 (ribosome binding site), 3) BBA_R0011 in pSB1A3 (lac I promoter), and 4) BBa_I0500 in pSB2K3 (pBAD/Arac promoter).  Made glycerol stocks for 1) to 4).  Minipreps 
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== Headline text ==
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6/18/09 Janice and Leigh: Did Qiagen maxipreps on double terminator, RBS pSB1A3, LacI promoter and pBAD promoter.
 
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DNA was isolated form all samples.
 

Revision as of 23:51, 16 September 2009


June 8, 2009 to June 15, 2009

Janice/Leigh: Made LB agar plates and liquid LB with tetracycline. Made tetracycline stock solution (5mg/ml).


June 16, 2009 to June 23, 2009

Janice/Leigh: Made LB liquid media and performed QIAGEN Plasmid Maxi Preps for 1) BBa_B0014 in pSB1AK3 (double terminator), 2) BBA_B0034 in pSB1A3 (ribosome binding site), 3) BBA_R0011 in pSB1A3 (lac I promoter), and 4) BBa_I0500 in pSB2K3 (pBAD/Arac promoter). Made glycerol stocks for 1) to 4). Minipreps


Headline text

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