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Team:Alberta/Project/Recombineering
From 2009.igem.org
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| - | Recombineering refers to the strategic use of recombination | + | Recombineering refers to the strategic use of recombination <i>in vivo</i> in order to reach a defined goal. In the case of BioBytes, a method is required to target the final construct to insertion at a specific place on the <i>E. coli</i> chromosome.</p> |
| - | To do this successfully | + | <P> |
| + | To do this successfully, three components must be taken into account: | ||
</p> | </p> | ||
| + | <P> - There must be a system for targeting the construct to a specific site for insertion </p> | ||
| + | <P> - Activation of the recombination system must be under experimenter control </p> | ||
| + | <P> - It must be possible to select for and verify colonies in which the insertion was successful </p> | ||
</font></div> | </font></div> | ||
Revision as of 20:44, 17 September 2009
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What is Recombineering?Recombineering refers to the strategic use of recombination in vivo in order to reach a defined goal. In the case of BioBytes, a method is required to target the final construct to insertion at a specific place on the E. coli chromosome. To do this successfully, three components must be taken into account: - There must be a system for targeting the construct to a specific site for insertion - Activation of the recombination system must be under experimenter control - It must be possible to select for and verify colonies in which the insertion was successful |
