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Template:Team:KULeuven/21 September 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
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# a new restriction digest on logA (1&2) was done. | # a new restriction digest on logA (1&2) was done. | ||
| - | # the re-enting of likA was succesfull and the liquid culture was miniprepped. likA: 179,3 ng/µl. a restriction digest with EcoRI and PstI was performed and put on gel. | + | # the re-enting of likA was succesfull and the liquid culture was miniprepped. likA: 179,3 ng/µl. a restriction digest with EcoRI and PstI was performed and put on gel. the signals were good. |
# a glycerol stock of ligY was made and some samples for sequencing were prepared. | # a glycerol stock of ligY was made and some samples for sequencing were prepared. | ||
| + | # a motherplate of LogA en LikA were made and liquid cultures to make a glycerol stock. | ||
| + | # liquid cultures were also made for empty top10 cells, standard promotor {{kulpart|BBa_I20260}} and LikA (blp and GFP in pSB3K3). | ||
Latest revision as of 15:22, 21 September 2009
- a new restriction digest on logA (1&2) was done.
- the re-enting of likA was succesfull and the liquid culture was miniprepped. likA: 179,3 ng/µl. a restriction digest with EcoRI and PstI was performed and put on gel. the signals were good.
- a glycerol stock of ligY was made and some samples for sequencing were prepared.
- a motherplate of LogA en LikA were made and liquid cultures to make a glycerol stock.
- liquid cultures were also made for empty top10 cells, standard promotor and LikA (blp and GFP in pSB3K3).
