Team:Illinois/SgrS

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Revision as of 20:53, 11 June 2009

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SgrS Target-GFP Fusion

People in Group: Francis Lee, Harsh Shah, Dave Korenchan

Purpose: We want to fuse the SgrS target sequence from the PtsG gene to the GFP gene on a low-copy plasmid and transform this into E. coli cells along with a high-copy plasmid carrying the small RNA SgrS gene. We expect to see translational repression of GFP by SgrS.

Protocol(s) Used: (links to protocols page)

Recipe(s) Used:

Primers Used:

June 12

Journal entry text goes here.