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Team:UC Davis/Approach
From 2009.igem.org
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| + | [[https://2009.igem.org/Team:UC_Davis/About_Us About Us]] [[https://2009.igem.org/Team:UC_Davis/Celiac Celiac Disease]] [[https://2009.igem.org/Team:UC_Davis/Treatments Current Treatment]] [[https://2009.igem.org/Team:UC_Davis/Approach Our Approach]] [[https://2009.igem.org/Team:UC_Davis/pH_Sensor pH Sensor]] [[https://2009.igem.org/Team:UC_Davis/pH_Sensor/parts pH Sensor Parts]] [[https://2009.igem.org/Team:UC_Davis/pH_Sensor/promoters pH Promoters]] [[https://2009.igem.org/Team:UC_Davis/Secretion/parts Secretion Parts]] | ||
Revision as of 20:18, 23 September 2009
Our Approach
We are going to break down gluten inside the stomach, because gliadin does not prompt an immune system response until absorption in the small intestines. Another worry is our delivery system taking residence somewhere outside the stomach, so we designed a pH inducible apoptosis system. This is achieved through sensing the change of pH from the stomach to the small intestine, a difference of about four to five.
Unlike other suggested treatments requiring the consumption of capsules or tablets, our method of using Escherichia coli is more cost- and time-efficient.
Induced Secretion
- Gene sequence for secretion system
- Adding Secretion
- Assay for measuring protein localization and activity
Sensing pH and Inducing Cell Death
- Finding an appropriate biological pH sensor
- Wiring the pH sensor into our system
- Assay for measuring the behavior of the pH sensor
[About Us] [Celiac Disease] [Current Treatment] [Our Approach] [pH Sensor] [pH Sensor Parts] [pH Promoters] [Secretion Parts]
