Team:Illinois/MicF

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Revision as of 15:22, 12 June 2009

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MicF Target-GFP Fusion

Purpose: MicF will be fused to the target sequence from the OmpF gene to the GFP gene on a low-copy plasmid and transform this into E. coli cells along with a high-copy plasmid carrying the MicF gene. We expect to see translational repression of GFP by the small RNA MicF.

Protocol(s) Used: (links to protocols page)

Recipe(s) Used:

Primers Used: