Team:BCCS-Bristol/Notebook/Week 6
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(New page: {{:Team:BCCS-Bristol/Header}} {{:Team:BCCS-Bristol/NotebookHeader}} ===Week 6=== * XL1-Blue transformations with ligations 1 and 2. * Restriction digests for FhuA and OsmE to prepare t...)
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(New page: {{:Team:BCCS-Bristol/Header}} {{:Team:BCCS-Bristol/NotebookHeader}} ===Week 6=== * XL1-Blue transformations with ligations 1 and 2. * Restriction digests for FhuA and OsmE to prepare t...)
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Revision as of 12:49, 25 September 2009
BCCS-Bristol
iGEM 2009
iGEM 2009
Week 6
- XL1-Blue transformations with ligations 1 and 2.
- Restriction digests for FhuA and OsmE to prepare their insertion downstream of the AraC-RBS costruct. Also GFP cut to fuse it with BOO14 terminator.
- Ligation set up between GFP and B0014 terminator (on pSB1AK3) and used to transform XL1-Blue.
- Transformations with AraC-RBS and pQE31-GFP didn't work. Repeated with NovaBlue cells.
- AraC-RBS transformations worked very well in contrast with pQE31-GFP and GFP-terminator that didn't work again.
- Repeat ligations between pQE31-GFP and GFP-terminator and transform XL1-Blue.
- Minipreps and Restr. Digests for AraC-RBS constructs.
- pQE31-GFP and GFP-terminator transformations failed.
- Ligations set up:
1. pQE31+GFP 2. AraC-RBS + FhuA 3. AraC-RBS + OsmE 4. GFP-terminator