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Team:Chiba/Notebook/Calendar/24 September 2009
From 2009.igem.org
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Yesterday's operation is [https://2009.igem.org/wiki/index.php?title=Team:Chiba/Notebook/Calendar/23_September_2009 here]. | Yesterday's operation is [https://2009.igem.org/wiki/index.php?title=Team:Chiba/Notebook/Calendar/23_September_2009 here]. | ||
Revision as of 13:03, 25 September 2009
(23_September_2009 <|>25_September_2009)
Examine limit of AHL generation
Yesterday's operation is here.
22:00
We measured OD.
OD600=1.98
Then, we spun down this culture solution and add 12.5 mL of flesh LB-Amp.
22:10
We add 12.5 μL of IPTG to the culture solution and started culture at 37 degrees Celsius.
