Team:Chiba/Notebook/Calendar/24 September 2009

From 2009.igem.org

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== Transformation(2)-1 ==
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*Plasmid
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plux-GFP(Cm, p15A)
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*Competent Cells
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JW1262(pCIA3-LuxR(Amp))
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JW1262(Null)
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21:35
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We transplanted these bacteria each plates.
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pCIA3-LuxR and plux-GFP --> LB-Amp, Cm plate
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 +
plux-GFP only --> LB-Cm plate
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21:50-
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We cultured these plates.

Revision as of 15:54, 25 September 2009

>Go to the Notebook page

(23_September_2009 <|>25_September_2009)


Examine limit of AHL generation

Yesterday's operation is here.


  • Today's operation

14:00

We made plates from each of mixture and 10 mL of LB-Amp, Cm solution medium.


  • Element of mixtures
Sample Namber 1 2 3 4 5 6 7 8
supernatant solution 5 mL --- --- --- --- --- --- 20 μL
1 --- 5 mL --- --- --- --- --- ---
2 --- --- 5 mL --- --- --- --- ---
3 --- --- --- 5 mL --- --- --- ---
4 --- --- --- --- 5 mL --- --- ---
5 --- --- --- --- --- 5 mL --- ---
6 --- --- --- --- --- --- 5 mL ---
LB-Amp,Cm 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL 9.8 mL
Total 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL


Transformation(2)-1

  • Plasmid

plux-GFP(Cm, p15A)


  • Competent Cells

JW1262(pCIA3-LuxR(Amp))

JW1262(Null)


21:35

We transplanted these bacteria each plates.

pCIA3-LuxR and plux-GFP --> LB-Amp, Cm plate

plux-GFP only --> LB-Cm plate


21:50-

We cultured these plates.

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