|
|
| Line 1: |
Line 1: |
| - | 1.colony check
| |
| - | ( sample No.) (no. of colonies)
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| - | 9 50<
| |
| - | 24 0
| |
| - | 29 0
| |
| - | 30 0
| |
| - | 31 8
| |
| - | 32 2
| |
| - |
| |
| - | 9 1
| |
| | | | |
| - | 2.digestion and ligation<br>
| |
| - |
| |
| - | digestion with restriction enzyme<br>
| |
| - |
| |
| - | '''K204024'''
| |
| - | <table border="1" Frame="box">
| |
| - | <tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
| |
| - | <tr><td>terminater(1-23L)</td><td>6</td><td>No. 14-A </td><td>12</td></tr>
| |
| - | <tr><td>EcoRI</td><td>1</td><td>EcoRI</td><td>1</td><tr>
| |
| - | <tr><td>XbaI</td><td>1</td><td>SpeI</td><td>1</td><tr>
| |
| - | <tr><td> No.2</td><td>2</td><td>No.2</td><td>2</td></tr>
| |
| - | <tr><td>dH<sub>2</sub>O</td><td>10</td><td>dH<sub>2</sub>O</td><td>4</td></tr>
| |
| - | <tr><td>total</td><td>20uL</td><td>total</td><td>20uL</td></tr>
| |
| - | </table>
| |
| - |
| |
| - |
| |
| - | '''K204029'''
| |
| - | <table border="1" Frame="box">
| |
| - | <tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
| |
| - | <tr><td>2-18F</td><td>6</td><td>2-16</td><td>10</td></tr>
| |
| - | <tr><td>EcoRI</td><td>1</td><td>EcoRI</td><td>1</td><tr>
| |
| - | <tr><td>XbaI</td><td>1</td><td>SpeI</td><td>1</td><tr>
| |
| - | <tr><td> No.2</td><td>2</td><td>No.2</td><td>2</td></tr>
| |
| - | <tr><td>dH<sub>2</sub>O</td><td>10</td><td>dH<sub>2</sub>O</td><td>6</td></tr>
| |
| - | <tr><td>total</td><td>20uL</td><td>total</td><td>20uL</td></tr>
| |
| - | </table>
| |
| - |
| |
| - |
| |
| - |
| |
| - | '''K204030'''
| |
| - | <table border="1" Frame="box">
| |
| - | <tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
| |
| - | <tr><td>2-18F</td><td>10</td><td>1-19B</td><td>6</td></tr>
| |
| - | <tr><td>SpeI</td><td>1</td><td>XbaI</td><td>1</td><tr>
| |
| - | <tr><td>PstI</td><td>1</td><td>PstI</td><td>1</td><tr>
| |
| - | <tr><td> No.2 </td><td>2</td><td>No.2</td><td>2</td></tr>
| |
| - | <tr><td>dH<sub>2</sub>O</td><td>6</td><td>dH<sub>2</sub>O</td><td>10</td></tr>
| |
| - | <tr><td>total</td><td>20uL</td><td>total</td><td>20uL</td></tr>
| |
| - | </table>
| |
| - |
| |
| - | ↓<br>
| |
| - | 37degree 3hr<br>
| |
| - | ↓<br>
| |
| - | Agarose Gel Electro-phoresis<br>
| |
| - | <br>
| |
| - | [[Image:Osaka090909.jpg]]<br>
| |
| - | No. 24 , 29 , 30 , 10-D , 10-E , ladder
| |
| - | ↓<br>
| |
| - | 16 degree O/N<br>
| |
| - |
| |
| - | 3.Min prep<br>
| |
| - | Result of Nanodrop concentration check
| |
| - | (sample No.) (concentration)
| |
| - | 1-1D 27.5
| |
| - | 10-D 85.1
| |
| - | 10-E 117.2
| |
| - |
| |
| - |
| |
| - | 4.transformation<br>
| |
| - | ligation sample No. 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40A ,40B
| |
| - | sample No. 24 , 29 , 30
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | 5.digestion and ligation<br>
| |
| - |
| |
| - | digestion with restriction enzyme<br>
| |
| - |
| |
| - |
| |
| - | '''K204041'''
| |
| - | <table border="1" Frame="box">
| |
| - | <tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
| |
| - | <tr><td>1-1D</td><td>12</td><td>1-12M</td><td>8</td></tr>
| |
| - | <tr><td>SpeI</td><td>1</td><td>XbaI</td><td>1</td><tr>
| |
| - | <tr><td>PstI</td><td>1</td><td>PstI</td><td>1</td><tr>
| |
| - | <tr><td> No.2 </td><td>2</td><td>No.2</td><td>2</td></tr>
| |
| - | <tr><td>dH<sub>2</sub>O</td><td>4</td><td>dH<sub>2</sub>O</td><td>8</td></tr>
| |
| - | <tr><td>total</td><td>20uL</td><td>total</td><td>20uL</td></tr>
| |
| - | </table>
| |
| - |
| |
| - |
| |
| - | '''K204042'''
| |
| - | <table border="1" Frame="box">
| |
| - | <tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
| |
| - | <tr><td>1-1D</td><td>12</td><td>1-8I</td><td>8</td></tr>
| |
| - | <tr><td>SpeI</td><td>1</td><td>XbaI</td><td>1</td><tr>
| |
| - | <tr><td>PstI</td><td>1</td><td>PstI</td><td>1</td><tr>
| |
| - | <tr><td> No.2 </td><td>2</td><td>No.2</td><td>2</td></tr>
| |
| - | <tr><td>dH<sub>2</sub>O</td><td>4</td><td>dH<sub>2</sub>O</td><td>8</td></tr>
| |
| - | <tr><td>total</td><td>20uL</td><td>total</td><td>20uL</td></tr>
| |
| - | </table>
| |
| - |
| |
| - | ↓<br>
| |
| - | 37degree 3hr<br>
| |
| - | ↓<br>
| |
| - | Agarose Gel Electro-phoresis<br>
| |
| - | <br>
| |
| - | [[Image:Osaka090909-2.jpg]]<br>
| |
| - | No. 41 , 42 , ladder
| |
| - | ↓<br>
| |
| - | 16 degree O/N<br>
| |
"
