Team:UNICAMP-Brazil/Notebooks/September 22

From 2009.igem.org

(Difference between revisions)
(ColiGuard)
(Electroelution verification)
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*The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
*The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
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*Now that the F plasmid was acquired, the next step is its recircularization.
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*Now that the F plasmid was obtained, the next step is its recircularization.
''Gabriel''
''Gabriel''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 20:21, 26 September 2009

ColiGuard

Electroelution verification

  • The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
  • Now that the F plasmid was obtained, the next step is its recircularization.

Gabriel