Team:UNICAMP-Brazil/Notebooks/September 22
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*The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified! | *The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified! | ||
- | *Now that the F plasmid was | + | *Now that the F plasmid was obtained, the next step is its recircularization. |
''Gabriel'' | ''Gabriel'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} |
Revision as of 20:21, 26 September 2009
ColiGuard
Electroelution verification
- The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
- Now that the F plasmid was obtained, the next step is its recircularization.
Gabriel