Team:UNICAMP-Brazil/Notebooks/September 21

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''Gabriel''
''Gabriel''
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====finO and finP's biobricks confirmation====
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* We repeated today the PCR's of the minipreps samples, once we now have received new Taq Polymerase enzymes.
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* We run an agarose gel of the reaction products and, according to it, we amplified in a few samples a fragment with the expected size of finO! We couldn't verify this amplification for finP though.
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* Nevertheless, we also got several inespecific amplification in almost all samples. We considered that this was caused by the increased amount of genomic DNA we obtained after the miniprep procedure.
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* Therefore, we will perform another miniprep of the cultures by which we best amplified our expected fragment.
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''Marcelo''
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Revision as of 20:48, 26 September 2009

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ColiGuard

Digested F plasmid's electroelution

  • The digested F plasmid (digested with HindIII) was electroeluted, according to protocol 9. A 110 V voltage was applied during 3h before its extraction from the membrane.
  • The next step is to verify if the electroelution went OK. The collected samples of purified plasmid will be applied to a 0,8% agarose gel.

Gabriel

finO and finP's biobricks confirmation

  • We repeated today the PCR's of the minipreps samples, once we now have received new Taq Polymerase enzymes.
  • We run an agarose gel of the reaction products and, according to it, we amplified in a few samples a fragment with the expected size of finO! We couldn't verify this amplification for finP though.
  • Nevertheless, we also got several inespecific amplification in almost all samples. We considered that this was caused by the increased amount of genomic DNA we obtained after the miniprep procedure.
  • Therefore, we will perform another miniprep of the cultures by which we best amplified our expected fragment.

Marcelo






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