"
August/23 August 2009
From 2009.igem.org
(Difference between revisions)
| Line 38: | Line 38: | ||
ligation sample | ligation sample | ||
⑤⑫⑬⑭⑮ | ⑤⑫⑬⑭⑮ | ||
| - | 2-18H | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118014 2-18H] |
| - | 1-8E | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R1051 1-8E] |
| - | 1-4E | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 1-4E] |
| - | 2-16N | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118006 2-16N] |
| - | 2-16L | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118005 2-16L] |
Revision as of 09:33, 29 September 2009
1.transformed check Checked the cell cultures transformed and plated out yesterday (8/22) for colony formation and made an approximate count of the number of colonies.
(ligation sample No.) (no. of colonies)
9 1
10 100~
11 100~
inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)
2.digestion and ligation
'8/22 digestion sample
↓
gel electrophoresis
gel cut
↓
purification by [QIAquick Nucleotide Removal Kit]
↓
ligation
ligation DNA 44 10* buffer 5 ligation 1 total 50uL
↓
16°C 3~4hr
↓
transfromation
3.transfromation
ligation sample ⑤⑫⑬⑭⑮ [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118014 2-18H] [http://partsregistry.org/wiki/index.php?title=Part:BBa_R1051 1-8E] [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 1-4E] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118006 2-16N] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118005 2-16L]
