Team:Newcastle/Meetings/16 April 2009

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Contents

Instructor Meeting 16 April 2009

Students: Jess, James, Hanny, Goksel Instructors:

Apologies: Craig, Matt, Jane, Arun

Agenda of the Meeting

  • More about flowchart/table/modelling
  • iGEM wiki changes
    • Europe Workshop: Most likely May 30/31 in London, UK at Imperial College.
    • Lethbridge - Bacteria over Batteries - bacterial battery that uses photosynthesis to generate electricity.
    • EPF-Lausanne - Brainstorm of 10 ideas.

Action Points of the Meeting

  • Need to show normal growth on diagram
  • Model population dynamics
  • Ask Nigel Robinson about Metallisation
  • Make powerpoint more detailed (biobrick slides)
    • Name promoters that we are going to use
    • Put them in the right order
  • Finding a homologue comK

Minutes of the Meeting

  • Knock out comK, and replace with one from another organism
  • Sigma D Motility, provides bi-stability
  • Work in a mutant background for biofilm formation, if we use sinI/sinR stochastic switch. Knocking out biofilm 'genes'
  • Use KinA
  • Methylase, no GFP. Instead of DNA rearrangement?
  • RNAs Restriction enzyme, instead of DNAase to prevent germination, but don't know how it will work
  • recA mutants, can repair themselves
  • Metallothiene incorporate with whole set of coat proteins of the spore
  • GFP and RFP with smtA to see where it binds on the coat of the spore
  • Knock out phosphotases rap. spo0E sporelation
  • Natural switches instead of synthetic ones
  • Streptococci Stochastisity Competence
  • Sigma F (spore specific) to control DNA rearrangement
  • Negative feedback to control smtA expression
  • Control mntH using mntR, also controls mntABCD, lets in Fe
  • Strong promoters - Sigma K, cotC, cotA - can be used for smtA expression
  • Sigma D is expressed all the time, provides bi-stability
  • May be able to use E-Coli biobricks for DNA rearrangement
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