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Team:UC Davis/Adding secretion
From 2009.igem.org
(Difference between revisions)
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style="border: 0px solid ; width: 78px; height: 37px;"></a> <a | style="border: 0px solid ; width: 78px; height: 37px;"></a> <a | ||
| - | href="https://2009.igem.org/Team:UC_Davis/Contacts_References"><img alt="" | + | href="https://2009.igem.org/Team:UC_Davis/Contacts_References"><img |
| - | src="https://static.igem.org/mediawiki/2009/1/1d/UCDAVIS_PIC7.png" | + | alt="" src="https://static.igem.org/mediawiki/2009/1/1d/UCDAVIS_PIC7.png" |
style="border: 0px solid ; width: 83px; height: 37px;"></a></big></big></big></big></span></b></div> | style="border: 0px solid ; width: 83px; height: 37px;"></a></big></big></big></big></span></b></div> | ||
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</span></big></span></b></p> | </span></big></span></b></p> | ||
<div style="text-align: center;"><b><span | <div style="text-align: center;"><b><span | ||
| - | style="font-size: 13.5pt; line-height: 115%; font-family: "Times New Roman","serif";" | + | style="font-size: 13.5pt; line-height: 115%; font-family: "Times New Roman","serif";"><span |
| - | style="text-decoration: underline;">Molecular data on some proteins</span | + | style="text-decoration: underline;">Molecular data on some proteins</span><o:p></o:p></span></b></div> |
<table class="MsoNormalTable" | <table class="MsoNormalTable" | ||
| - | style="border: medium none ; border-collapse: collapse; text-align: left; margin-left: auto; margin-right: auto; width: | + | style="border: medium none ; border-collapse: collapse; text-align: left; margin-left: auto; margin-right: auto; width: 87px; height: 38px;" |
border="1" cellpadding="0" cellspacing="0"> | border="1" cellpadding="0" cellspacing="0"> | ||
<tbody> | <tbody> | ||
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<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";"><o:p> </o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><o:p> <br> |
| + | </o:p></span></b></p> | ||
| + | <div style="text-align: center;"> | ||
| + | <div style="text-align: center;"><b><span | ||
| + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><o:p>Name</o:p></span></b><br> | ||
| + | </div> | ||
| + | <b><span | ||
| + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><o:p></o:p></span></b></div> | ||
</td> | </td> | ||
<td | <td | ||
| Line 182: | Line 189: | ||
valign="top" width="157"><small><small> </small></small> | valign="top" width="157"><small><small> </small></small> | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
| - | style="margin-bottom: 0.0001pt; line-height: normal;"><small><small><small><small><b><span | + | style="margin-bottom: 0.0001pt; line-height: normal;"><small><small><small><small><small><small><small><b><span |
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";">Molecular | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><small>Molecular</small></small><br> |
| - | Weight</span></b></small></small></small></small></p> | + | <small><small>Weight</small></small></span></b></small></small></small></small></small></small></small></p> |
<small><small> </small></small></td> | <small><small> </small></small></td> | ||
<td | <td | ||
style="border-style: solid solid solid none; border-color: black black black -moz-use-text-color; border-width: 1pt 1pt 1pt medium; padding: 0in 5.4pt; width: 117.5pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | style="border-style: solid solid solid none; border-color: black black black -moz-use-text-color; border-width: 1pt 1pt 1pt medium; padding: 0in 5.4pt; width: 117.5pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | ||
| - | valign="top" width="157"><small> </small> | + | valign="top" width="157"><small><small> </small></small> |
<p class="MsoNormal" | <p class="MsoNormal" | ||
| - | style="margin-bottom: 0.0001pt; line-height: normal;"><small><b><span | + | style="margin-bottom: 0.0001pt; line-height: normal;"><small><small><b><span |
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>Number | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><small>Number</small> |
| - | of residues</small><o:p></o:p></span></b></small></p> | + | <small>of residues</small></small><o:p></o:p></span></b></small></small></p> |
| - | <small> </small></td> | + | <small><small> </small></small></td> |
<td | <td | ||
style="border-style: solid solid solid none; border-color: black black black -moz-use-text-color; border-width: 1pt 1pt 1pt medium; padding: 0in 5.4pt; width: 118.25pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | style="border-style: solid solid solid none; border-color: black black black -moz-use-text-color; border-width: 1pt 1pt 1pt medium; padding: 0in 5.4pt; width: 118.25pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | ||
| - | valign="top" width="158"> | + | valign="top" width="158"><small> |
| + | </small> | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
| - | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | + | style="margin-bottom: 0.0001pt; line-height: normal;"><small><b><span |
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>< | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><small>Number |
| - | Number | + | of polypeptide</small></small><o:p></o:p></span></b></small></p> |
| - | of polypeptide</small><o:p></o:p></span></b></p> | + | <small></small></td> |
| - | </td> | + | |
</tr> | </tr> | ||
<tr style=""> | <tr style=""> | ||
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<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>Cytochrome | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><small>Cytochrome |
| - | <i>c </i>(human | + | <i>c </i>(human</small></small></span></b></p> |
</td> | </td> | ||
<td | <td | ||
style="border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 117.9pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | style="border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 117.9pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | ||
| - | valign="top" width="157"><small> </small> | + | valign="top" width="157"><small><small> </small></small> |
<p class="MsoNormal" | <p class="MsoNormal" | ||
| - | style="margin-bottom: 0.0001pt; line-height: normal;"><small><b><span | + | style="margin-bottom: 0.0001pt; line-height: normal;"><small><small><b><span |
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";">13,000<o:p></o:p></span></b></small></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>13,000</small><o:p></o:p></span></b></small></small></p> |
| - | <small> </small></td> | + | <small><small> </small></small></td> |
<td | <td | ||
style="border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 117.5pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | style="border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 117.5pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | ||
| - | valign="top" width="157"> | + | valign="top" width="157"><small> |
| + | </small> | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
| - | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | + | style="margin-bottom: 0.0001pt; line-height: normal;"><small><b><span |
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";">104<o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>104</small><o:p></o:p></span></b></small></p> |
| - | </td> | + | <small></small></td> |
<td | <td | ||
style="border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 118.25pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | style="border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 118.25pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | ||
| Line 232: | Line 240: | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";">1<o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>1</small><o:p></o:p></span></b></p> |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 241: | Line 249: | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>Ribonuclease | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><small>Ribonuclease |
| - | A(bovine pancreas)</small><o:p></o:p></span></b></p> | + | A(bovine pancreas)</small></small><o:p></o:p></span></b></p> |
</td> | </td> | ||
<td | <td | ||
| Line 249: | Line 257: | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";">13,700<o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><br> |
| + | 13,700</small><o:p></o:p></span></b></p> | ||
</td> | </td> | ||
<td | <td | ||
| Line 256: | Line 265: | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";">124<o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>124</small><o:p></o:p></span></b></p> |
</td> | </td> | ||
<td | <td | ||
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<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";">1<o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>1</small><o:p></o:p></span></b></p> |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 272: | Line 281: | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>Serum | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><small>Serum |
| - | albumin (human)</small><o:p></o:p></span></b></p> | + | albumin (human)</small></small><o:p></o:p></span></b></p> |
</td> | </td> | ||
<td | <td | ||
| Line 280: | Line 289: | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";">68,500<o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><br> |
| + | 68,500</small><o:p></o:p></span></b></p> | ||
</td> | </td> | ||
<td | <td | ||
| Line 287: | Line 297: | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";">609<o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>609</small><o:p></o:p></span></b></p> |
</td> | </td> | ||
<td | <td | ||
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<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
| - | style="font-size: 14pt; font-family: "Times New Roman","serif";">1<o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>1</small><o:p></o:p></span></b></p> |
</td> | </td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
| - | <p style="text-align: center;" class="MsoNormal"><b><span | + | <p style="text-align: center;" class="MsoNormal"><font size="-1"><b><span |
| - | style="font-size: 13.5pt; line-height: 115%; font-family: "Times New Roman","serif";">(Page | + | style="font-size: 13.5pt; line-height: 115%; font-family: "Times New Roman","serif";"><small><small>(Page |
128, third edition “Lehninger | 128, third edition “Lehninger | ||
| - | Principles of Biochemistry” by David L. Nelson and Michael M. Cox)</span></b><b><span | + | Principles of Biochemistry” by David L. Nelson and Michael M. Cox)</small></small></span></b></font><b><span |
| - | style="font-size: 14pt; line-height: 115%; font-family: "Times New Roman","serif";"><o:p> | + | style="font-size: 14pt; line-height: 115%; font-family: "Times New Roman","serif";"><o:p><small><small><small><small><small><small><small> |
| - | <br> | + | </small></small></small></small></small></small></small><br> |
</o:p></span></b></p> | </o:p></span></b></p> | ||
<p style="text-align: center;" class="MsoNormal"><b><span | <p style="text-align: center;" class="MsoNormal"><b><span | ||
Revision as of 23:01, 1 October 2009
Adding Secretion:
General
model for secretion system:
Secretion Models:
Click on a specific model for more information:
Advantages
of having several models:
In
our secretion system, we are using two genes with different sizes as
our target
secretion genes, GFP being short in length and Luciferase being
comparably long
would test our secretion system and its ability to secrete small and
large
proteins.
Testing multiple different combinations;
Signal
Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone,
would
help us find the best combination for our secretion system based on
their
secretion ability and rank them from strongest to weakest in strength
(in this
specific system).
Secretion Models:
Click on a specific model for more information:
There
is wide range of genes present in our environment. Therefore, it is
important
to measure our secretion system’s ability to secrete genes of various
sizes.
Molecular data on some proteins
|
|
Molecular |
Number
of residues |
Number
of polypeptide |
|
Cytochrome c (human |
13,000 |
104 |
1 |
|
Ribonuclease
A(bovine pancreas) |
|
124 |
1 |
|
Serum
albumin (human) |
|
609 |
1 |
(Page
128, third edition “Lehninger
Principles of Biochemistry” by David L. Nelson and Michael M. Cox)
