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Team:Chiba/Notebook/Calendar/29 September 2009
From 2009.igem.org
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== Gel electrophoresis == | == Gel electrophoresis == | ||
| - | Yesterday's oparation is [https://2009.igem.org/ | + | Yesterday's oparation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/28_September_2009 here]. |
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Gel electrophoresis again(135 V, 27 min) | Gel electrophoresis again(135 V, 27 min) | ||
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== Digestion Test == | == Digestion Test == | ||
Revision as of 07:48, 3 October 2009
(28_September_2009 <|>30_September_2009)
Contents |
Gel electrophoresis
Yesterday's oparation is here.
- Today's operation
10:40-
Mini Prep.
13:30-
Gel electrophoresis(135 V, 27 min)
---DNA Clean & Concentrator---
15:50-
Gel electrophoresis again(135 V, 27 min)
Digestion Test
- Sample
Vector(plux-GFP)
18:55-
Gel electrophoresis(135 V, 25 min)
19:45
Took a picture
Transformation
- Sample
Competent Cells : XL10G(K)
Plasmid : plux-GFP(Cm)
21:45-
CmのプレートにまいてCultured it at 37 degrees Celsius.
Digestion
- Sample
Vector : plux-GFP(p15A)
Insert : sfGFP
Enzymes : Nco1, BamH1
21:17
We added Enzymes and took it 37 degrees Celsius.
---3 h---
We took it 65 degrees Celsius for 酵素を失活させる
---20 min---
24:42
We kept it in refrigerator.
PCR
- Template
plux-HSUTK
