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EPF-Lausanne/22 September 2009
From 2009.igem.org
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- RO2 + BB double transformatnts grew. #8 is pink, #3 is white and # 7 is pink. | - RO2 + BB double transformatnts grew. #8 is pink, #3 is white and # 7 is pink. | ||
| + | ===Trp K.O. strains=== | ||
| + | After about 35-40 hours. | ||
| + | |||
| + | 2 out of 3 grew : JW 4356-2 0.14, JRG 1046 0.00, JRG 465 : 0.04... | ||
| + | |||
| + | We will let them more time at 18°C... maybe it will grow a bit more !! | ||
| + | |||
| + | ===New cultures=== | ||
| + | '''TrpR K.O.''' | ||
| + | |||
| + | -JW4356-2 (KANA) | ||
| + | |||
| + | -JRG1046 | ||
| + | |||
| + | - JRG 465 | ||
| + | |||
| + | All the 3 in 3ml of SOB, at 37°C. We took them from the glycerol stock. | ||
| + | |||
| + | ===Cultures for characterization=== | ||
| + | IPTG : stock solution of 100 mM. Induction at 1 mM should be enough, so we have to dilute it 100x. | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 13:18, 4 October 2009
Contents |
Wet Lab
Results of the cultures after one more night
- RO1 + BB double transformants grew (white).
- RO2 #4,5,10 didn't grow.
- RO1 #1,2 didn't grow.
- RO2 + BB double transformatnts grew. #8 is pink, #3 is white and # 7 is pink.
Trp K.O. strains
After about 35-40 hours.
2 out of 3 grew : JW 4356-2 0.14, JRG 1046 0.00, JRG 465 : 0.04...
We will let them more time at 18°C... maybe it will grow a bit more !!
New cultures
TrpR K.O.
-JW4356-2 (KANA)
-JRG1046
- JRG 465
All the 3 in 3ml of SOB, at 37°C. We took them from the glycerol stock.
Cultures for characterization
IPTG : stock solution of 100 mM. Induction at 1 mM should be enough, so we have to dilute it 100x.
People in the lab
Basile
