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EPF-Lausanne/24 September 2009
From 2009.igem.org
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Revision as of 14:10, 4 October 2009
Wet Lab
Transformation in Trp K.O.
The 3 newly competent strains (JW4356-2, JRG 1046, JRG 465) will be transformed with :
- RO2#4 + BB1 (+Amp, Kana) -> double transformation.
- RO2#4 (+Kana)
- RO1#1 (+Kana)
- RO1#1 + BB1 (+Amp, Kana) -> double transformation.
Note : the 3 control (1 ug/ul DNA, 100 pg/ul DNA, 10 pg/ul DNA -> 10ul for each transf) + KANA. We will count the nb of clones the next morning on the plates to determine transformation efficiency.
People in the lab
Mél, Nicolas, Christian, Basile
