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Team:Illinois/MicC
From 2009.igem.org
(Difference between revisions)
(New page: {{UIUC09Temp}} {{Illinois}} == '''MicC Target-GFP Fusion''' == '''Purpose:''' '''Protocol(s) Used:''' (links to protocols page) '''Recipe(s) Used:''' '''Primers Used:''' == '''June...) |
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== '''MicC Target-GFP Fusion''' == | == '''MicC Target-GFP Fusion''' == | ||
| - | '''Purpose:''' | + | '''People in Group:''' Phil Bell, Sonam Tanna, Davel Patel |
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| + | '''Purpose:''' We want to fuse the MicC target sequence from the OmpC gene to the GFP gene on a low-copy plasmid and transform this into E. coli cells along with a high-copy plasmid carrying the MicC gene. We expect to see translational repression of GFP by the small RNA MicC. | ||
'''Protocol(s) Used:''' (links to protocols page) | '''Protocol(s) Used:''' (links to protocols page) | ||
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'''Primers Used:''' | '''Primers Used:''' | ||
| - | == '''June | + | == '''June 15''' == |
| + | |||
| + | PCRs of sRNA genes and target sequences were performed using E. coli extracted chromosomal DNA and primers. We were able to purify the ompC gene on a 3% agarose gel, however no band was visible from the micC PCR product. | ||
| - | + | == '''June 16''' == | |
| + | Another PCR of the micC gene was done. | ||
Revision as of 08:39, 17 June 2009
MicC Target-GFP Fusion
People in Group: Phil Bell, Sonam Tanna, Davel Patel
Purpose: We want to fuse the MicC target sequence from the OmpC gene to the GFP gene on a low-copy plasmid and transform this into E. coli cells along with a high-copy plasmid carrying the MicC gene. We expect to see translational repression of GFP by the small RNA MicC.
Protocol(s) Used: (links to protocols page)
Recipe(s) Used:
Primers Used:
June 15
PCRs of sRNA genes and target sequences were performed using E. coli extracted chromosomal DNA and primers. We were able to purify the ompC gene on a 3% agarose gel, however no band was visible from the micC PCR product.
June 16
Another PCR of the micC gene was done.
