"
Team:Heidelberg/k4l1um
From 2009.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
'''SH''': | '''SH''': | ||
| - | * [[Team:Heidelberg/n4tr1um#Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)]]Miniprep of GFP template plasmid, pcDNA5/FRT | + | * [[Team:Heidelberg/n4tr1um#Isolation of plasmid DNA by alkaline lysis (mini and maxiprep) Miniprep]]Miniprep of GFP template plasmid, pcDNA5/FRT |
{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
|- | |- | ||
Revision as of 13:38, 17 June 2009
6-15-2009
Lab: LV, SH, CZ
SH:
- Team:Heidelberg/n4tr1um#Isolation of plasmid DNA by alkaline lysis (mini and maxiprep) MiniprepMiniprep of GFP template plasmid, pcDNA5/FRT
| Nr | pcDNA5/FRT | GFP |
|---|---|---|
| 1 | 4,7 | 39,7 |
| 2 | 5,9 | 14,9 |
| 3 | 3,7 | 3,8 |
| 4 | 5,0 | 7,8 |
- Maxiprep pcDNA/FRT 188.7ng/µL
LV:
- Extraction of CMV promoter from 2008 distribution
- Transformation of DH5a cell with CMV promoter
- portzughtr
6-16-2009
Lab: LV, SH, CZ
- Annealing of oligos (JeT, cFos, Min)
- Purification of cDNA via 2% / 3% agarosegel
- Site directed mutagenesis of pcDNA5/FRT
- Dpn1 digestion
- Transformation of DH5a
