Team:Washington/Accomplishments

From 2009.igem.org

(Difference between revisions)
Line 30: Line 30:
- Make more universal, to work with all proteins
- Make more universal, to work with all proteins
 +
- Optimize secretion-tag and nano-tag for fusion proteins
- Optimize secretion-tag and nano-tag for fusion proteins
Line 59: Line 60:
- Optimize RBS's  
- Optimize RBS's  
 +
- Move secretion sequence into new plasmid, not TetR
- Move secretion sequence into new plasmid, not TetR
 +
- Try variety of cell lines.  
- Try variety of cell lines.  
Line 85: Line 88:
- Use new CDS display vector to display streptavidin
- Use new CDS display vector to display streptavidin
 +
- Design monomeric protein to bind peptide sequence  
- Design monomeric protein to bind peptide sequence  
 +
- Utilize other proteins on CDS to test for activity of other proteins  
- Utilize other proteins on CDS to test for activity of other proteins  

Revision as of 17:11, 6 October 2009

Home The Team Project Description Notebook User Guide Goals and Accomplishments


Target structure.png

BBa_K215000

BBa_K215001

BBa_K215002

BBa_K215010

BBa_K215011

BBa_K215090

BBa_K215091

Current Status: Complete

Future directions:

- Make more universal, to work with all proteins

- Optimize secretion-tag and nano-tag for fusion proteins

Secretion structure.png

BBa_K215100

BBa_K215101

BBa_K215102

BBa_K215103

BBa_K215104

BBa_K215105

BBa_K215106

BBa_K215107

BBa_K215108

BBa_K215109

Current Status: In progress

Future directions:

- Optimize RBS's

- Move secretion sequence into new plasmid, not TetR

- Try variety of cell lines.

Display structure.png

BBa_K215200

BBa_K215201

BBa_K215210

BBa_K215211

BBa_K215250

BBa_K215251

BBa_K215260

BBa_K215261

Current Status: In progress

Future Directions:

- Use new CDS display vector to display streptavidin

- Design monomeric protein to bind peptide sequence

- Utilize other proteins on CDS to test for activity of other proteins

Biobricks Submitted Current Status Report Future Directions


Quick overview of what was done and the status of everyting. Maybe 3 paragraphs (display, secretion, conclusions)