"
Team:Illinois/MicA
From 2009.igem.org
(Difference between revisions)
(New page: {{UIUC09Temp}} {{Illinois}} == '''MicA Target-GFP Fusion''' == '''Purpose:''' '''Protocol(s) Used:''' (links to protocols page) '''Recipe(s) Used:''' '''Primers Used:''' == '''June...) |
(→June 12) |
||
| Line 15: | Line 15: | ||
Journal entry text goes here. | Journal entry text goes here. | ||
| + | |||
| + | == '''June 16'''== | ||
| + | |||
| + | We used PCR to extract the sRNA gene: MicA and target sequence : ompA. from the e.coli chromosome. We then ran a gel to make sure that we had the right DNA fragments. Our results corresponded to our predictions. | ||
| + | |||
| + | [[Image:Example.jpg]] | ||
| + | |||
| + | =='''June 17'''== | ||
| + | |||
| + | We are completing a digestion of MicA, OmpA, and OmpF. Following the digestion we will incubate with SAP and then run a gel to verify the digestion. We will then extract the DNA for the sRNA target sequence and sRNA gene. | ||
Revision as of 16:16, 17 June 2009
Contents |
MicA Target-GFP Fusion
Purpose:
Protocol(s) Used: (links to protocols page)
Recipe(s) Used:
Primers Used:
June 12
Journal entry text goes here.
June 16
We used PCR to extract the sRNA gene: MicA and target sequence : ompA. from the e.coli chromosome. We then ran a gel to make sure that we had the right DNA fragments. Our results corresponded to our predictions.
June 17
We are completing a digestion of MicA, OmpA, and OmpF. Following the digestion we will incubate with SAP and then run a gel to verify the digestion. We will then extract the DNA for the sRNA target sequence and sRNA gene.
