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Team:UNIPV-Pavia/Methods Materials/Absorbance
From 2009.igem.org
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| - | == <html><font class="dayw_style">Absorbance and | + | == <html><font class="dayw_style">Absorbance and dilutions in liquid growing medium</font></html> == |
| - | The proportionality between absorbance measurement (OD600) and colture's | + | The proportionality between absorbance measurement (OD600) and colture's dilutions was verified, confirming the possibility of using OD600 to measure the bacterial quantity in the well, regardless of the total volume present in the well. |
This experiment was done in two different growing mediums, LB and M9, and in three different total volumes in the well, 100μl, 200μl and 300μl. | This experiment was done in two different growing mediums, LB and M9, and in three different total volumes in the well, 100μl, 200μl and 300μl. | ||
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| - | [[Image: | + | [[Image:Unipv_asb_dilution_LB.jpg| 500px|Absorbance and colture's dilutions in LB|thumb|center|Absorbance and colture's dilutions in LB]] |
| - | [[Image: | + | [[Image:unipv_asb_dilution_M9.jpg| 500px|Absorbance and colture's dilutions in M9|thumb|center|Absorbance and colture's dilutions in M9]] |
Revision as of 13:46, 8 October 2009
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Measurements - Absorbance |
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Absorbance and water dispensationWe find no relevant differences between the measures before and after the dispensation of different volumes of water in the well, both for bacterial coltures and simple liquid growing medium. You can find more informations about the experiment at [http://aimed11.unipv.it/iGEM2009 Download Protocol] - Test n.9, 07/07/09.
Absorbance and volumeThe proportionality between absorbance (OD600) measurement and volume of colture in the well was verified. You can find more informations about the experiment at [http://aimed11.unipv.it/iGEM2009 Download Protocol] - Test n.9, 07/07/09.
Absorbance and dilutions in liquid growing mediumThe proportionality between absorbance measurement (OD600) and colture's dilutions was verified, confirming the possibility of using OD600 to measure the bacterial quantity in the well, regardless of the total volume present in the well. This experiment was done in two different growing mediums, LB and M9, and in three different total volumes in the well, 100μl, 200μl and 300μl. You can find more informations about the experiments at [http://aimed11.unipv.it/iGEM2009 Download Protocol] - Test n.20, 13/08/09 and Test n.20 "M9", 28/08/09.
File:Unipv asb dilution LB.jpg Absorbance and colture's dilutions in LB File:Unipv asb dilution M9.jpg Absorbance and colture's dilutions in M9
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