"
Team:Tsinghua/Experiment1
From 2009.igem.org
(Difference between revisions)
GuoQiangChen (Talk | contribs) (→Synthesis of the Genome of GenSniper) |
GuoQiangChen (Talk | contribs) (→Bottom-Up Approach) |
||
| Line 3: | Line 3: | ||
In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s). | In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s). | ||
| - | Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, | + | Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, pET-28a and pACYCDuet-1. |
[[Image:pET28a.jpg|450px|pET28a]] | [[Image:pET28a.jpg|450px|pET28a]] | ||
[[Image:pACYCDuet1.jpg|450px|pACYCDuet1]] | [[Image:pACYCDuet1.jpg|450px|pACYCDuet1]] | ||
| + | |||
| + | We incorporate the segment from Nu1 to B on bacteriophage lambda genome into pET-28a while clone the left segment from C to FII (C will be further engineered) into pACYCDuet-1. Before cotranformation with therapeutic DNA, we firstly cotranform the two plasmids encoding the GenSniper genome into BL21 DE3 and evaluate their function. | ||
[[Image:BUA001.jpg|800px|center|BUA001]] | [[Image:BUA001.jpg|800px|center|BUA001]] | ||
Revision as of 15:52, 9 October 2009
Contents |
Synthesis of the Genome of GenSniper
Bottom-Up Approach
In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s).
Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, pET-28a and pACYCDuet-1.
We incorporate the segment from Nu1 to B on bacteriophage lambda genome into pET-28a while clone the left segment from C to FII (C will be further engineered) into pACYCDuet-1. Before cotranformation with therapeutic DNA, we firstly cotranform the two plasmids encoding the GenSniper genome into BL21 DE3 and evaluate their function.
Top-Down Approach
