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Team:Todai-Tokyo/Notebook/bread
From 2009.igem.org
(Difference between revisions)
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*PCR of gpd1 promoter<BR> | *PCR of gpd1 promoter<BR> | ||
| + | =='''October'''== | ||
| + | *PCR of Glu1<BR> | ||
| + | *estriction enzyme fragmentation of mtlD by XbaI and PstI | ||
Revision as of 03:55, 13 October 2009
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Plan
Insert mtlD(mannitol synthetase) in the yeast chromosome by homologous recombination
Replace gpd gene by mtlD
September
- PCR of mtlD
- TA cloning of mtlD
- PCR of gpd1 promoter
October
- PCR of Glu1
- estriction enzyme fragmentation of mtlD by XbaI and PstI
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