Team:IPN-UNAM-Mexico/Notebook/July 7 2009

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Revision as of 06:35, 13 October 2009

Digestion of the following Biobricks with the indicated enzimes:

Biobrick	Order	Enzime
R0079	Prefix	E, S
F1610	Sufix	E, X
B0034	Prefix	E, S
C0078	Sufix	E, X
B0034	Prefix	E, S
C0079	Sufix	E, X
B0015	Sufix	E, X

Note: E=EcoR I, S=Spe I, X=Xba I

Cuts were done thinking on the standard assembling 10 with the 2-way-ligation.

Procedement:

- Take the purified DNA stocks from the previous Biobricks
- Take the enzimes and add 100 μl of Buffer

2 μl of each sample were analized on a Spectrophotometer to determine the concentration of DNA on each Biopart.

Biobrick	Concentration (ng/μl)
F1610	158.9
B0015	11.5
C0079	7.5
B0034	11.6
C0078	7.7
B0079	11.2

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                                      <p align="left">&nbsp;</p>
                                      <p align="left"><br>
+                                    </p>
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                                      <p align="left">&nbsp;</p>
-<p align="left" class="style8">Cuts were done thinking on the standard assembling 10  with the 2-way-ligation.</p>
+                                    <p align="left" class="style8">Cuts were done thinking on the standard assembling 10  with the 2-way-ligation.</p>
                                      <p align="left" class="style8">&nbsp;</p>
                                      <p align="left" class="style8"><strong>Procedement:</strong></p>
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-                                     <p align="left" class="style8">&nbsp;</p>
+                                     <p align="left" >&nbsp;</p>
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+                                     <p align="left" >&nbsp;</p>
-                                     <p align="left">...</p>
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+                                        <td width="50%"><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Notebook"><img src="https://static.igem.org/mediawiki/2009/3/38/AnteriorMX.JPG" alt="Previous" width="70" height="70" align="right" border="0"></a> </td>
+                                        <td width="50%" height="104"><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Notebook/July_8_2009#July_8th"> <img src="https://static.igem.org/mediawiki/2009/3/3a/SiguienteMX.JPG" alt="Next" width="70" height="70" align="left" border="0"></a></td>
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+                                    <p align="left">&nbsp;</p>
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+                                  <p align="left">&nbsp;</p>
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