"
Team:Washington/Project/Opda
From 2009.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
|} | |} | ||
| + | =Opda= | ||
| + | |||
| + | ==Background== | ||
Opda is an organophosphate-degrading enzyme from Agrobacterium radiobacter. It is capable of degrading a wide range of organophosphates, most notably pesticides that are poisonous to humans, such as paraoxon. We chose to biobrick this enzyme for a number of reasons. First and foremost, this enzyme is easy to assay for since it can hydrolyze substrates (e.g. paraoxon) and form a bright yellow product. This yellow product would make it easy to see that the Opda was present and functioning in our system. And secondly, Opda is a very useful enzyme that could have applications in future iGEM and other synthetic biology projects, so its presence in the Standard Registry of Biological Parts is beneficial. | Opda is an organophosphate-degrading enzyme from Agrobacterium radiobacter. It is capable of degrading a wide range of organophosphates, most notably pesticides that are poisonous to humans, such as paraoxon. We chose to biobrick this enzyme for a number of reasons. First and foremost, this enzyme is easy to assay for since it can hydrolyze substrates (e.g. paraoxon) and form a bright yellow product. This yellow product would make it easy to see that the Opda was present and functioning in our system. And secondly, Opda is a very useful enzyme that could have applications in future iGEM and other synthetic biology projects, so its presence in the Standard Registry of Biological Parts is beneficial. | ||
| + | |||
| + | ==Experiments== | ||
Revision as of 06:59, 13 October 2009
| Target Vector | Secretion System | Display System |
|---|
Opda
Background
Opda is an organophosphate-degrading enzyme from Agrobacterium radiobacter. It is capable of degrading a wide range of organophosphates, most notably pesticides that are poisonous to humans, such as paraoxon. We chose to biobrick this enzyme for a number of reasons. First and foremost, this enzyme is easy to assay for since it can hydrolyze substrates (e.g. paraoxon) and form a bright yellow product. This yellow product would make it easy to see that the Opda was present and functioning in our system. And secondly, Opda is a very useful enzyme that could have applications in future iGEM and other synthetic biology projects, so its presence in the Standard Registry of Biological Parts is beneficial.
==Experiments==
