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Team:Todai-Tokyo/Notebook/bread
From 2009.igem.org
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=='''October'''== | =='''October'''== | ||
*PCR of Glu1<BR> | *PCR of Glu1<BR> | ||
| - | * | + | *cut mtlD by XbaI and PstI<BR> |
| + | *PCR of gpd1 promoter with ExTaq<BR> | ||
Revision as of 08:23, 13 October 2009
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Plan
Insert mtlD(mannitol synthetase) in the yeast chromosome by homologous recombination
Replace gpd gene by mtlD
September
- PCR of mtlD
- TA cloning of mtlD
- PCR of gpd1 promoter
October
- PCR of Glu1
- cut mtlD by XbaI and PstI
- PCR of gpd1 promoter with ExTaq
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