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Team:Todai-Tokyo/Notebook/bread
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=='''Plan'''== | =='''Plan'''== | ||
| - | + | '''Aim:'''Create yeast that makes sweet and low energy sugar from starch | |
| + | |||
| + | '''Methods:'''<BR> | ||
| + | #Clone the glucoamylase gene from Saccharomycopsis fibuligera<BR> | ||
| + | #Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination<BR> | ||
Replace gpd gene by mtlD<BR> | Replace gpd gene by mtlD<BR> | ||
Revision as of 08:34, 13 October 2009
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Plan
Aim:Create yeast that makes sweet and low energy sugar from starch
Methods:
- Clone the glucoamylase gene from Saccharomycopsis fibuligera
- Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination
Replace gpd gene by mtlD
September
- PCR of mtlD
- TA cloning of mtlD
- PCR of gpd1 promoter
October
- PCR of Glu1
- cut mtlD by XbaI and PstI
- PCR of gpd1 promoter with ExTaq
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