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Team:Todai-Tokyo/Notebook/bread
From 2009.igem.org
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{{:Team:Todai-Tokyo/Template}} | {{:Team:Todai-Tokyo/Template}} | ||
=='''Plan'''== | =='''Plan'''== | ||
| - | '''Aim:'''Create yeast that | + | '''Aim:'''Create yeast that can be used to make sweet and low energy bread<BR> |
'''Methods:'''<BR> | '''Methods:'''<BR> | ||
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*PCR of mtlD<BR> | *PCR of mtlD<BR> | ||
*TA cloning of mtlD<BR> | *TA cloning of mtlD<BR> | ||
| - | *PCR of gpd1 promoter<BR> | + | *PCR of gpd1 promoter with Pfu Ultra<BR> |
=='''October'''== | =='''October'''== | ||
*PCR of Glu1<BR> | *PCR of Glu1<BR> | ||
| + | *TA cloning of Glu1<BR> | ||
*cut mtlD by XbaI and PstI<BR> | *cut mtlD by XbaI and PstI<BR> | ||
*PCR of gpd1 promoter with ExTaq<BR> | *PCR of gpd1 promoter with ExTaq<BR> | ||
| + | |||
Revision as of 08:41, 13 October 2009
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Protocols | Ethics |
|---|
Plan
Aim:Create yeast that can be used to make sweet and low energy bread
Methods:
- Clone the glucoamylase gene from Saccharomycopsis fibuligera
- Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination
- Replace gpd gene by mtlD
September
- PCR of mtlD
- TA cloning of mtlD
- PCR of gpd1 promoter with Pfu Ultra
October
- PCR of Glu1
- TA cloning of Glu1
- cut mtlD by XbaI and PstI
- PCR of gpd1 promoter with ExTaq
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Protocols | Ethics |
|---|
