Team:Chiba/Project
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=== Constructing A Flow of Activation === | === Constructing A Flow of Activation === | ||
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Revision as of 01:10, 14 October 2009
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E.coli Time Manager Since 2008
IntroductionIt has been demanded in biological engineering that making the function that organisms sense the passage of time. We can utilize the excellent functions of organisms such as material synthesis or sensing as a device for drug delivery system or physical exam when these functions have been time-controlled as we like. However we can control cells individually, without method to control them all together, advantage of excellent functions may attenuate. Then we want to make the timer that cells works concurrently. Project DesignFor making some timers without need of synchronizer system and that can be used in a single system we try to make following design.
複数細胞の同時コントロール2008 Melbourne, 2007/2008 Paris, and 2008 NYMU dealed in a bacterial timer but in these project it needs the synchronizing system to make the behavior of cell population synchronized. Then we have aimed at a making of the function that can control a mass of cells all together without synchronizing system. We have used cell-cell signaling system for this. By using it, massive numbers of cells in the system can share the time they fell.
Signaling SystemIn this project, we use acylated homoserine lactones (AHLs), signaling molecules used for [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] in gram negative bacteria. Senders express LuxI or similar enzymes, which catalyze the production of AHLs, under the control of a constitutive (Tet) promoter. Each cell thus generates AHL more or less at a constant rate. AHL can freely permeate cell membranes and are detected by neighboring cells. Receivers constitutively express LuxR proteins (or a similar ortholog), the protein that detects AHL concentrations. When AHLs bind LuxR proteins, the AHL-LuxR complex activates the Lux promoter. The threshold [AHL] at which switching occurs is determined by the affinity of AHL for the particulr LuxR ortholog. about quorum sensing) Constructing A Delay Switch (Since 2008)We are constructing delay switches to control/preset the timing of target gene expression. Our project uses two classes of bacteria: senders and receivers. Senders produce signaling molecules, and receivers are activated only after a particular concentration of this molecule is reached. The combinatorial use of senders/receivers allows us to make a‘switching consortium’which activates different genes at the preset times. Constructing A Delay Switch, Multiple WaysIn principle, there are three ways to delay the activation of chemical communications;
Inter-species communications! We decided to go for the strategy inspired by Japanese-classic experience; Whenever we speak to somebody in English, we often experience a certain delay in activating the communication. We though this is exactly what we pursued in. See Exp #4. Same applies to the reverse, too. When somebody speaks to us, we definitely need some time (sometime infinite) to get activated. This is all in spite that he/ she was loud and clear enough. The less affinity (perception) we have to English, the longer we need to activate them.See exp #5. Constructing A Flow of Activation
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