"
Team:Todai-Tokyo/Notebook/bread
From 2009.igem.org
(Difference between revisions)
(→9/22) |
|||
| Line 17: | Line 17: | ||
=='''9/22'''== | =='''9/22'''== | ||
| - | *read the sequence of | + | *read the sequence of mtlD→successful<BR> |
| - | + | ||
=='''9/23'''== | =='''9/23'''== | ||
Revision as of 03:46, 14 October 2009
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Protocols | Ethics |
|---|
Contents |
Plan
Aim:Create yeast that can be used to make sweet and low energy bread
Methods:
- Clone the glucoamylase gene from Saccharomycopsis fibuligera
- Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination
- Replace gpd gene by mtlD
~9/20
- PCR of mtlD
- PCR of gpd1 promoter
- TA cloning of mtlD
"'9/21
- Miniprep of mtlD
9/22
- read the sequence of mtlD→successful
9/23
- PCR of gpd1 promoter with Pfu Ultra
October
- PCR of Glu1
- TA cloning of Glu1
- cut mtlD by XbaI and PstI
- PCR of gpd1 promoter with ExTaq
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Protocols | Ethics |
|---|
