Team:Freiburg bioware/cloning

From 2009.igem.org

(Difference between revisions)
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GATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTC
GATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTC
GCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
GCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
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</td>
</td>
</tr>
</tr>
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<br>
<br>
<br>
<br>
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 +
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</div><a name="23"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
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</div>
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<div class="art-PostContent">
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<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 +
 +
<table border="5">
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<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/4/47/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg" name="Strep+FluA+longlinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+longlinker+Foki in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 +
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 +
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 +
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 +
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCT
 +
GGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA
 +
ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA
 +
ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG
 +
AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC
 +
AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG
 +
CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT
 +
GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA
 +
CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT
 +
TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT
 +
CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA
 +
ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG
 +
AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 +
 +
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 +
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 +
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRI
 +
LEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQAD
 +
EMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNG
 +
AVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 +
<br><br></p></td></table>
 +
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/c/ce/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.txt">Strep+FluA+longlinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 +
 +
</div><a name="23"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 +
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d8/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+middellinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 +
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 +
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 +
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 +
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
 +
TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
 +
GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
 +
ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
 +
TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTA
 +
TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCT
 +
ATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGT
 +
TATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATG
 +
GTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGA
 +
GCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCAT
 +
AAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGG
 +
CGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTA
 +
AATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
 +
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 +
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 +
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
 +
VMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
 +
YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLS
 +
VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 +
 +
<br><br></p></td></table>
 +
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/4/48/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.txt">Strep+FluA+middellinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 +
 +
</div><a name="23"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 +
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d8/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+middellinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 +
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 +
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 +
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 +
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
 +
TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
 +
GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
 +
ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
 +
TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTA
 +
TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCT
 +
ATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGT
 +
TATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATG
 +
GTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGA
 +
GCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCAT
 +
AAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGG
 +
CGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTA
 +
AATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
 +
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 +
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 +
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
 +
VMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
 +
YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLS
 +
VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 +
 +
<br><br></p></td></table>
 +
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/4/48/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.txt">Strep+FluA+middellinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 +
 +

Revision as of 08:29, 14 October 2009

FREiGEM

Index


*His+FluA+Split+Foki_pMA
*His+Dig+Split+FokA_pMA
* DsbA+His+Dig+Split+FokA_pMA
* Strep+FluA+ShortLinker+FokI_pMA
* His+FluA+middellinker+FokA_pMA
* His+FluA+middellinker+Foki_pMA
* His+FluA+shortlinker+FokA_pMA
* His+FluA+shortlinker+Foki_pMA
* His+Dig+longlinker+FokA_pMA
* His+Dig+longlinker+Foki_pMA
* Strep+FluA+Split+Foki_pMA
* His+Dig+middellinker+FokA_pMA
* His+Dig+middellinker+Foki_pMA
* His+Dig+shortlinker+FokA_pMA
* His+Dig+shortlinker+Foki_pMA
* His+Dig+Split+Foki_pMA
* His+FluA+longlinker+FokA_pMA
* His+FluA+longlinker+Foki_pMA
* His+FluA+Split+FokA_pMA
* Strep+Dig+longlinker+FokA_pMA
* Strep+Dig+longlinker+Foki_pMA
* Strep+Dig+middellinker+FokA_pMA

 Parts

 His+FluA+Split+Foki_pMA

back to index

His+FluA+SplitLinker+Foki in pMA Vector

 nucleotid sequence of ORF-1:
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

AA sequence of ORF-1:

MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

Commented GenBank file

  • His+FluA+Split+Foki_pMA.txt



    •  His+Dig+Split+FokA_pMA

    back to index

    His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+FokA_pMA.txt



    •  DsbA+His+Dig+Split+FokA_pMA

    back to index

    DsbA+His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGMKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF NNGEINFTG

    Commented GenBank file

  • DsbA+His+Dig+Split+FokA_pMA.txt



    •  Strep+FluA+ShortLinker+FokI_pMA

    back to index

    Strep+FluA+ShortLinker+FokI in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA TCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI GGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+ShortLinker+FokI_pMA.txt



    •  His+FluA+middellinker+FokA_pMA

    back to index

    His+FluA+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+FokA_pMA.txt



    •  His+FluA+middellinker+Foki_pMA

    back to index

    His+FluA+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+Foki_pMA.txt



    •  His+FluA+shortlinker+FokA_pMA

    back to index

    His+FluA+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA CCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE MIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+shortlinker+FokA_pMA.txt



    •  His+FluA+shortlinker+Foki_pMA

    back to index

    His+FluA+shortlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC CGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG CGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTG GTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAAC AAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCAC CGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAG CCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTG AGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCT GACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTA CCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY GYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRN KHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGE MIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+shortlinker+Foki_pMA.txt



    •  His+Dig+longlinker+FokA_pMA

    back to index

    His+Dig+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+longlinker+FokA_pMA.txt



    •  His+Dig+longlinker+Foki_pMA

    back to index

    His+Dig+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCG ATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCG TAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTG TCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTAT AAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGT GCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCA CCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLP IGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHI TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+longlinker+Foki_pMA.txt



    •  Strep+FluA+Split+Foki_pMA

    back to index

    Strep+FluA+Split+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+Split+Foki_pMA.txt



    •  His+Dig+middellinker+FokA_pMA

    back to index

    His+Dig+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+middellinker+FokA_pMA.txt



    •  His+Dig+middellinker+Foki_pMA

    back to index

    His+Dig+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC AGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCT GATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCT GAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCA AATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTG ACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGA GGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGG AAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQA DEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+middellinker+Foki_pMA.txt



    •  His+Dig+shortlinker+FokA_pMA

    back to index

    His+Dig+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCAT TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAG CCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAG CGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGA ATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCG TGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAAC CATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGAT TGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCC GTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM KVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQ RYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVL SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+shortlinker+FokA_pMA.txt



    •  His+Dig+shortlinker+Foki_pMA

    back to index

    His+Dig+shortlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCAT TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAG CGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAA CGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGA ATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCG TGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAAC CATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGAT CGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCC GTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM KVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEME RYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVL SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+shortlinker+Foki_pMA.txt



    •  His+Dig+Split+Foki_pMA

    back to index

    His+Dig+Split+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCG TATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACG TTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTG AGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCA TATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCG GTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGT AAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKA YSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+Foki_pMA.txt



    •  His+FluA+longlinker+FokA_pMA

    back to index

    His+FluA+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT CGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATAC CGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATA GCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTAT GTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTG GAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCG GCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAA ACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGG TGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAAT TCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY VKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSV EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+longlinker+FokA_pMA.txt



    •  His+FluA+longlinker+Foki_pMA

    back to index

    His+FluA+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT CGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATAC CGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATT CTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTAT GTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTG GAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCG GCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATC ACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGG AGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAAT TCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV MEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERY VEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSV EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+longlinker+Foki_pMA.txt



    •  His+FluA+Split+FokA_pMA

    back to index

    His+FluA+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTG CCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCA AGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAG TGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAG ATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACC CCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAA CTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACT GGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCC AAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCA GAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACA CCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATG AATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEG KSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYII GYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACK VNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+Split+FokA_pMA.txt



    •  Strep+Dig+longlinker+FokA_pMA

    back to index

    Strep+Dig+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA TCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCC CGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTAT AACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAA CCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATC CGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAA GGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAA TGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCA AAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGG GAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGY NLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL NHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+longlinker+FokA_pMA.txt



    •  Strep+Dig+longlinker+Foki_pMA

    back to index

    Strep+Dig+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA TCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCC CGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTAT AATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAA TCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATC CGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAA GGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAA TGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCA AAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGC GAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGY NLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL NHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+longlinker+Foki_pMA.txt



    •  Strep+Dig+middellinker+FokA_pMA

    back to index

    Strep+Dig+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC AATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNL PIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH KTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+middellinker+FokA_pMA.txt



    •  Strep+Dig+middellinker+Foki_pMA

    back to index

    Strep+Dig+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG TGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCG ATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTG CCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGAC CCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA GTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAAC TATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGC AGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAG GCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATC AATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNL PIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH ITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+middellinker+Foki_pMA.txt



    •  Strep+FluA+Split+FokA_pMA

    back to index

    Strep+FluA+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+middellinker+Foki_pMA.txt



    •  Strep+FluA+Split+FokA_pMA

    back to index

    Strep+FluA+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+Split+FokA_pMA.txt



    •  Strep+Dig+shortlinker+FokA_pMA

    back to index

    Strep+Dig+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA ACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA TCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAG GCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACA CATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGT TCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAG CTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGT AGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACAC TGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGT TAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD RILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQ ADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNS NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+shortlinker+FokA_pMA.txt



    •  Strep+Dig+shortlinker+Foki_pMA

    back to index

    Strep+Dig+shortlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA ACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA TCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAG GCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACA TCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGT TCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAG CTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGT TGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTC TGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGT TAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD RILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQ ADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNS NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+shortlinker+Foki_pMA.txt



    •  Strep+Dig+Split+FokA_pMA

    back to index

    Strep+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTG CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACC AAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGAT GCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGA ACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTG TTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCT GAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGC TGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTT CGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDT KAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNY KAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+Split+FokA_pMA.txt



    •  Strep+Dig+Split+Foki_pMA

    back to index

    Strep+Dig+Split+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTG CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACA AAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGAT GGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGA ACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTG TTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCT GAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGC TGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTT CGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVAT KAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNY KAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+Split+Foki_pMA.txt



    •  Strep+FluA+longlinker+FokA_pMA

    back to index

    Strep+FluA+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACC GGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAA ATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTA CCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTC TATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGC CATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCA AAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATG CAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAA CGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGT TCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTG AACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCT GATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTC GCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILE MKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEM QRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAV LSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+longlinker+FokA_pMA.txt



    •  Strep+FluA+longlinker+Foki_pMA

    back to index

    Strep+FluA+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCT GGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRI LEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQAD EMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNG AVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+longlinker+Foki_pMA.txt



    •  Strep+FluA+middellinker+FokA_pMA

    back to index

    Strep+FluA+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTA TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCT ATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGT TATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATG GTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGA GCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCAT AAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGG CGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTA AATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK VMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLS VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+middellinker+FokA_pMA.txt



    •  Strep+FluA+middellinker+FokA_pMA

    back to index

    Strep+FluA+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTA TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCT ATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGT TATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATG GTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGA GCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCAT AAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGG CGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTA AATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK VMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLS VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+middellinker+FokA_pMA.txt





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