Team:Freiburg bioware/cloning

From 2009.igem.org

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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
</div>
</div>
<div class="art-PostContent">
<div class="art-PostContent">
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<td align="center">
<td align="center">
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<img src="https://static.igem.org/mediawiki/2009/thumb/b/b9/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg" name="Strep+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
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<img src="https://static.igem.org/mediawiki/2009/thumb/d/db/Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.jpg" name="Strep+FluA+shortlinker+FokA in pMA Vector" width="480" height="480" />
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<br><p align="center">Strep+FluA+middellinker+Foki in pMA Vector</p>
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<br><p align="center">Strep+FluA+shortlinker+FokA in pMA Vector</p>
</td>
</td>
<td align="left">
<td align="left">
Line 2,506: Line 2,506:
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
-
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
+
AAGTGAACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAG
-
TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
+
GAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTA
-
GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
+
TATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGG
-
ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
+
AAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAA
-
TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTA
+
CATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTC
-
TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGT
+
CCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTT
-
ATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGT
+
ATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAG
-
TATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATG
+
AACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTA
-
GTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGA
+
TCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTA
-
GCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCAT
+
AAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGC
-
ATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGG
+
AATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGAT
-
TGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTA
+
CAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATG
-
AATTCAACAATGGCGAGATCAATTTTACCGGTTAA
+
GGGAGATCAATTTTACCGGTTAA
-
 
+
-
 
+
</td>
</td>
Line 2,532: Line 2,530:
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
-
AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
+
AACKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEF
-
VMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMER
+
FMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKE
-
YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLS
+
NQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEEL
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VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
LIGGEMIKAGTLTLEEVRRKFNNGEINFTG
-
 
+
Line 2,542: Line 2,539:
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/a/a3/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.txt">Strep+FluA+middellinker+Foki_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/4/49/Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.txt">Strep+FluA+shortlinker+FokA_pMA.txt</a></li>
<br>
<br>
<br>
<br>

Revision as of 08:39, 14 October 2009

FREiGEM

Index


*His+FluA+Split+Foki_pMA
*His+Dig+Split+FokA_pMA
* DsbA+His+Dig+Split+FokA_pMA
* Strep+FluA+ShortLinker+FokI_pMA
* His+FluA+middellinker+FokA_pMA
* His+FluA+middellinker+Foki_pMA
* His+FluA+shortlinker+FokA_pMA
* His+FluA+shortlinker+Foki_pMA
* His+Dig+longlinker+FokA_pMA
* His+Dig+longlinker+Foki_pMA
* Strep+FluA+Split+Foki_pMA
* His+Dig+middellinker+FokA_pMA
* His+Dig+middellinker+Foki_pMA
* His+Dig+shortlinker+FokA_pMA
* His+Dig+shortlinker+Foki_pMA
* His+Dig+Split+Foki_pMA
* His+FluA+longlinker+FokA_pMA
* His+FluA+longlinker+Foki_pMA
* His+FluA+Split+FokA_pMA
* Strep+Dig+longlinker+FokA_pMA
* Strep+Dig+longlinker+Foki_pMA
* Strep+Dig+middellinker+FokA_pMA

 Parts

 His+FluA+Split+Foki_pMA

back to index

His+FluA+SplitLinker+Foki in pMA Vector

 nucleotid sequence of ORF-1:
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

AA sequence of ORF-1:

MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

Commented GenBank file

  • His+FluA+Split+Foki_pMA.txt



    •  His+Dig+Split+FokA_pMA

    back to index

    His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+FokA_pMA.txt



    •  DsbA+His+Dig+Split+FokA_pMA

    back to index

    DsbA+His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGMKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF NNGEINFTG

    Commented GenBank file

  • DsbA+His+Dig+Split+FokA_pMA.txt



    •  Strep+FluA+ShortLinker+FokI_pMA

    back to index

    Strep+FluA+ShortLinker+FokI in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA TCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI GGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+ShortLinker+FokI_pMA.txt



    •  His+FluA+middellinker+FokA_pMA

    back to index

    His+FluA+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+FokA_pMA.txt



    •  His+FluA+middellinker+Foki_pMA

    back to index

    His+FluA+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+Foki_pMA.txt



    •  His+FluA+shortlinker+FokA_pMA

    back to index

    His+FluA+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA CCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE MIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+shortlinker+FokA_pMA.txt



    •  His+FluA+shortlinker+Foki_pMA

    back to index

    His+FluA+shortlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC CGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG CGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTG GTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAAC AAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCAC CGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAG CCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTG AGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCT GACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTA CCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY GYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRN KHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGE MIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+shortlinker+Foki_pMA.txt



    •  His+Dig+longlinker+FokA_pMA

    back to index

    His+Dig+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+longlinker+FokA_pMA.txt



    •  His+Dig+longlinker+Foki_pMA

    back to index

    His+Dig+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCG ATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCG TAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTG TCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTAT AAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGT GCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCA CCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLP IGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHI TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+longlinker+Foki_pMA.txt



    •  Strep+FluA+Split+Foki_pMA

    back to index

    Strep+FluA+Split+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+Split+Foki_pMA.txt



    •  His+Dig+middellinker+FokA_pMA

    back to index

    His+Dig+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+middellinker+FokA_pMA.txt



    •  His+Dig+middellinker+Foki_pMA

    back to index

    His+Dig+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC AGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCT GATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCT GAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCA AATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTG ACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGA GGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGG AAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQA DEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+middellinker+Foki_pMA.txt



    •  His+Dig+shortlinker+FokA_pMA

    back to index

    His+Dig+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCAT TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAG CCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAG CGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGA ATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCG TGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAAC CATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGAT TGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCC GTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM KVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQ RYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVL SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+shortlinker+FokA_pMA.txt



    •  His+Dig+shortlinker+Foki_pMA

    back to index

    His+Dig+shortlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCAT TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAG CGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAA CGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGA ATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCG TGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAAC CATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGAT CGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCC GTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM KVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEME RYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVL SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+shortlinker+Foki_pMA.txt



    •  His+Dig+Split+Foki_pMA

    back to index

    His+Dig+Split+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCG TATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACG TTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTG AGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCA TATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCG GTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGT AAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKA YSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+Foki_pMA.txt



    •  His+FluA+longlinker+FokA_pMA

    back to index

    His+FluA+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT CGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATAC CGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATA GCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTAT GTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTG GAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCG GCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAA ACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGG TGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAAT TCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY VKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSV EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+longlinker+FokA_pMA.txt



    •  His+FluA+longlinker+Foki_pMA

    back to index

    His+FluA+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT CGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATAC CGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATT CTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTAT GTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTG GAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCG GCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATC ACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGG AGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAAT TCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV MEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERY VEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSV EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+longlinker+Foki_pMA.txt



    •  His+FluA+Split+FokA_pMA

    back to index

    His+FluA+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTG CCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCA AGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAG TGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAG ATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACC CCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAA CTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACT GGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCC AAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCA GAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACA CCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATG AATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEG KSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYII GYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACK VNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+Split+FokA_pMA.txt



    •  Strep+Dig+longlinker+FokA_pMA

    back to index

    Strep+Dig+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA TCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCC CGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTAT AACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAA CCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATC CGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAA GGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAA TGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCA AAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGG GAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGY NLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL NHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+longlinker+FokA_pMA.txt



    •  Strep+Dig+longlinker+Foki_pMA

    back to index

    Strep+Dig+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA TCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCC CGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTAT AATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAA TCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATC CGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAA GGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAA TGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCA AAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGC GAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGY NLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL NHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+longlinker+Foki_pMA.txt



    •  Strep+Dig+middellinker+FokA_pMA

    back to index

    Strep+Dig+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC AATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNL PIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH KTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+middellinker+FokA_pMA.txt



    •  Strep+Dig+middellinker+Foki_pMA

    back to index

    Strep+Dig+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG TGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCG ATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTG CCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGAC CCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA GTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAAC TATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGC AGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAG GCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATC AATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNL PIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH ITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+middellinker+Foki_pMA.txt



    •  Strep+FluA+Split+FokA_pMA

    back to index

    Strep+FluA+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+middellinker+Foki_pMA.txt



    •  Strep+FluA+Split+FokA_pMA

    back to index

    Strep+FluA+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+Split+FokA_pMA.txt



    •  Strep+Dig+shortlinker+FokA_pMA

    back to index

    Strep+Dig+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA ACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA TCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAG GCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACA CATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGT TCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAG CTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGT AGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACAC TGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGT TAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD RILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQ ADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNS NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+shortlinker+FokA_pMA.txt



    •  Strep+Dig+shortlinker+Foki_pMA

    back to index

    Strep+Dig+shortlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA ACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA TCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAG GCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACA TCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGT TCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAG CTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGT TGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTC TGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGT TAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD RILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQ ADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNS NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+shortlinker+Foki_pMA.txt



    •  Strep+Dig+Split+FokA_pMA

    back to index

    Strep+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTG CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACC AAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGAT GCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGA ACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTG TTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCT GAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGC TGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTT CGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDT KAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNY KAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+Split+FokA_pMA.txt



    •  Strep+Dig+Split+Foki_pMA

    back to index

    Strep+Dig+Split+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTG CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACA AAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGAT GGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGA ACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTG TTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCT GAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGC TGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTT CGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVAT KAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNY KAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+Split+Foki_pMA.txt



    •  Strep+FluA+longlinker+FokA_pMA

    back to index

    Strep+FluA+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACC GGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAA ATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTA CCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTC TATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGC CATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCA AAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATG CAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAA CGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGT TCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTG AACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCT GATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTC GCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILE MKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEM QRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAV LSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+longlinker+FokA_pMA.txt



    •  Strep+FluA+longlinker+Foki_pMA

    back to index

    Strep+FluA+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCT GGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRI LEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQAD EMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNG AVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+longlinker+Foki_pMA.txt



    •  Strep+FluA+middellinker+FokA_pMA

    back to index

    Strep+FluA+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTA TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCT ATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGT TATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATG GTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGA GCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCAT AAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGG CGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTA AATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK VMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLS VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+middellinker+FokA_pMA.txt



    •  Strep+FluA+middellinker+Foki_pMA

    back to index

    Strep+FluA+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTA TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGT ATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGT TATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATG GTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGA GCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCAT ATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGG TGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTA AATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK VMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMER YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLS VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+middellinker+Foki_pMA.txt



    •  Strep+FluA+shortlinker+FokA_pMA

    back to index

    Strep+FluA+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAG GAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTA TATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGG AAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAA CATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTC CCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTT ATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAG AACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTA TCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTA AAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGC AATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGAT CAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATG GGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEF FMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKE NQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEEL LIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+shortlinker+FokA_pMA.txt





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