Team:Washington/Notebook/Ni-column

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(New page: ====Ni-column Set up==== #Transfer 2mL NiNTA beads (Quigen) to column #wash column with 10mL dH2O #Equillibrate column with 10mL running buffer (usually PBS) #To reuse column ##Wash with ...)
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Revision as of 04:08, 15 October 2009

Ni-column Set up

  1. Transfer 2mL NiNTA beads (Quigen) to column
  2. wash column with 10mL dH2O
  3. Equillibrate column with 10mL running buffer (usually PBS)
  4. To reuse column
    1. Wash with 12mL dH2O
    2. Wash with 12mL 100mM EDTA
    3. Wash with 12mL dH2O
    4. wash with 12mL 100mM Ni(SO4)
    5. Wash with 12mL dH2O
    6. Add 12mL 20% Ethanol run till ~5mL remains in column
    7. cap for use later
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