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User contributions
From 2009.igem.org
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- 13:55, 21 October 2009 (diff | hist) N IBB Pune/23 June 2009 (New page: {{Team:IBB_Pune/header}} {{Team:IBB_Pune/menu}})
- 13:55, 21 October 2009 (diff | hist) IBB Pune/20 June 2009 (top)
- 13:55, 21 October 2009 (diff | hist) IBB Pune/19 June 2009 (top)
- 13:55, 21 October 2009 (diff | hist) IBB Pune/18 June 2009 (top)
- 13:54, 21 October 2009 (diff | hist) IBB Pune/17 June 2009 (top)
- 13:54, 21 October 2009 (diff | hist) IBB Pune/16 June 2009 (top)
- 13:54, 21 October 2009 (diff | hist) IBB Pune/15 June 2009 (top)
- 13:54, 21 October 2009 (diff | hist) IBB Pune/14 June 2009 (top)
- 13:54, 21 October 2009 (diff | hist) IBB Pune/13 June 2009 (top)
- 13:54, 21 October 2009 (diff | hist) IBB Pune/12 June 2009 (top)
- 13:53, 21 October 2009 (diff | hist) IBB Pune/5 June 2009 (top)
- 13:53, 21 October 2009 (diff | hist) IBB Pune/11 June 2009 (top)
- 13:53, 21 October 2009 (diff | hist) IBB Pune/10 June 2009 (top)
- 13:53, 21 October 2009 (diff | hist) IBB Pune/9 June 2009 (top)
- 13:53, 21 October 2009 (diff | hist) IBB Pune/8 June 2009 (top)
- 13:52, 21 October 2009 (diff | hist) IBB Pune/7 June 2009 (top)
- 13:52, 21 October 2009 (diff | hist) IBB Pune/6 June 2009 (top)
- 13:52, 21 October 2009 (diff | hist) IBB Pune/6 June 2009
- 13:52, 21 October 2009 (diff | hist) IBB Pune/4 June 2009 (top)
- 13:51, 21 October 2009 (diff | hist) IBB Pune/3 June 2009 (top)
- 13:51, 21 October 2009 (diff | hist) IBB Pune/2 June 2009 (top)
- 13:50, 21 October 2009 (diff | hist) IBB Pune/1 June 2009 (top)
- 13:50, 21 October 2009 (diff | hist) IBB Pune/29 May 2009 (top)
- 13:50, 21 October 2009 (diff | hist) IBB Pune/28 May 2009 (top)
- 13:49, 21 October 2009 (diff | hist) IBB Pune/26 May 2009 (top)
- 13:49, 21 October 2009 (diff | hist) IBB Pune/15 May 2009 (top)
- 13:47, 21 October 2009 (diff | hist) N IBB Pune/22 June 2009 (New page: I) Gel elution of thick PCR bands. Double digestion of both. With ES and XP. Gel- Bands at *4.2 *4.4 YAY!! Double digestion successful...)
- 13:42, 21 October 2009 (diff | hist) N IBB Pune/21 June 2009 (New page: I) Transformation with PCR product, dilute DNA 1:10 and use 4ul. NO Transformants :-( Why? * Dilution? * Linear DNA, try ligation before transformation. * Kan conc too high? II) Ligat...)
- 13:38, 21 October 2009 (diff | hist) N IBB Pune/20 June 2009 (New page: I) Ran a preparative gel. *pGFP = 330ng/ul *pBR322 = 120 ng/ul II) PCR of CCR5 with P1 and P2 . No bands in Gel. Backtracking reveals a calculation mistake. Using more primer and hig...)
- 13:32, 21 October 2009 (diff | hist) N IBB Pune/19 June 2009 (New page: I) Gel elution. (Kit used, Bangalore Genei) A280 of eluted DNA *0.0138 *0.0123 *0.0134 *0.0365 ~500 ng DNA ! Too good to be true, running a gel to confirm. II) pBR322 isolated succes...)
- 13:27, 21 October 2009 (diff | hist) N IBB Pune/18 June 2009 (New page: I) Birboin and Dolly protocol for plasmid isolation followed. Cool picture perfect bands!! Gel slices cut for elution... II) Transformation with sigma kit using NEB10b and DB3.1. III) ...)
- 13:21, 21 October 2009 (diff | hist) N IBB Pune/17 June 2009 (New page: I) Test PCR of Heme Oxygenase Biobrick, entire template used, with 200pg primer and annealing temp reduced to 53.9. Other program left constant. Result No bands seen, except for positive...)
- 11:27, 21 October 2009 (diff | hist) Team:IBB Pune/BIOETHICS
- 07:25, 21 October 2009 (diff | hist) Team:IBB Pune/Parts (→Parts shipped to the registry)
- 07:21, 21 October 2009 (diff | hist) Team:IBB Pune/Parts (→Parts designed by us)
- 07:20, 21 October 2009 (diff | hist) Team:IBB Pune/Parts (→Parts shipped to the registry)
- 07:09, 21 October 2009 (diff | hist) Template:Team:IBB Pune/footer
- 07:03, 21 October 2009 (diff | hist) Team:IBB Pune/Parts (→Parts shipped to the registry)
- 06:59, 21 October 2009 (diff | hist) Team:IBB Pune/Parts (→Parts shipped to the registry)
- 06:47, 21 October 2009 (diff | hist) Team:IBB Pune/BIOETHICS
- 14:43, 19 October 2009 (diff | hist) IBB Pune/8 October 2009 (top)
- 14:42, 19 October 2009 (diff | hist) IBB Pune/8 October 2009
- 14:41, 19 October 2009 (diff | hist) IBB Pune/8 October 2009
- 14:41, 19 October 2009 (diff | hist) IBB Pune/8 October 2009
- 14:39, 19 October 2009 (diff | hist) N IBB Pune/8 October 2009 (New page: I] Assembly. Digestions:- ES XP Constitutive promoter LacO Promoter RBS mRFP YcdB RBS ...)
- 13:08, 19 October 2009 (diff | hist) N File:Insbb logo priv.JPG (ibb logo) (top)
- 08:31, 18 October 2009 (diff | hist) Team:IBB Pune/Parts (→Parts Submitted to the Registry)
- 08:27, 18 October 2009 (diff | hist) Team:IBB Pune/Parts (→Parts Submitted to the Registry)
- 08:23, 18 October 2009 (diff | hist) Team:IBB Pune/Parts (→Parts Submitted to the Registry)
- 07:57, 18 October 2009 (diff | hist) Team:IBB Pune/Parts (→Parts Submitted to the Registry)
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