User contributions
From 2009.igem.org
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- 09:15, 21 October 2009 (diff | hist) N Week21 (New page: 1. Specific growth rates determined at different conc. of Lactose for ptet A (SR1) and plac K(SR4) 0.1g/l 0.5g/l 1g/l 1.5g/l 3.5g/l 2. Along with varying Lactose conc., exp...)
- 09:15, 21 October 2009 (diff | hist) N Week22 (New page: 1. Specific growth rates determined at different conc. of Lactose for plac A (SR2) and ptet K(SR3) 0.1g/l 0.5g/l 1g/l 1.5g/l 3.5g/l 2. Along with varying Lactose conc., exp...)
- 09:10, 21 October 2009 (diff | hist) Week19
- 09:10, 21 October 2009 (diff | hist) N Week20 (New page: 1. Specific growth rates determined at different conc. of Lactose for plac A (SR2) and ptet K(SR3) 0.1g/l 0.5g/l 1g/l 1.5g/l 3.5g/l 2. Beta galactosidase expression determi...)
- 09:08, 21 October 2009 (diff | hist) N Week19 (New page: 1. Specific growth rates determined at different conc. of Lactose 0.1g/l 0.5g/l 1g/l 1.5g/l 3.5g/l 2. Beta galactosidase expression determined.)
- 09:05, 21 October 2009 (diff | hist) Team:IIT Bombay India/Notebook
- 09:05, 21 October 2009 (diff | hist) N Week18 (New page: 1. Growth curve for specific growth rate determination of all the four strains on Minimal medium.)
- 09:04, 21 October 2009 (diff | hist) Week17
- 09:04, 21 October 2009 (diff | hist) Week16 (top)
- 09:04, 21 October 2009 (diff | hist) Week15
- 09:04, 21 October 2009 (diff | hist) Week14
- 08:55, 21 October 2009 (diff | hist) N Week17 (New page: 1. Growth curve for specific growth rate determination of all the four strains on Minimal medium.)
- 08:54, 21 October 2009 (diff | hist) N Week16 (New page: 1. FACS for YFP at different IPTG concentrations.)
- 08:52, 21 October 2009 (diff | hist) N Week15 (New page: 1. Performed the time course, at the intervals of every 2 hours for four strains at different IPTG conc. 2.Sample preparation for FACS 3.Actual FACS data generation. 4.Analysis of FACS ...)
- 08:46, 21 October 2009 (diff | hist) Team:IIT Bombay India/Notebook
- 08:43, 21 October 2009 (diff | hist) Team:IIT Bombay India/Notebook
- 08:35, 21 October 2009 (diff | hist) N Week14 (New page: 1. Prepared antibiotic and IPTG stocks, media for time course experiments with the construct.)
- 08:33, 21 October 2009 (diff | hist) Week13
- 08:31, 21 October 2009 (diff | hist) N Week13 (New page: 1. Preparation for transformations and actual transformations done for the four plasmids in ''E.coli'' MG1655)
- 05:33, 21 October 2009 (diff | hist) Team:IIT Bombay India/Notebook
- 05:12, 21 October 2009 (diff | hist) N Week12 (New page: 1.FACS with Host as Control 2.Transformations done with new host strain ''E.coli'' MG1655- some problem with ampicillin plates.)
- 09:16, 24 July 2009 (diff | hist) Team:IIT Bombay India
- 09:00, 24 July 2009 (diff | hist) N Week9 (New page: 1. Cultured Host strain)
- 08:59, 24 July 2009 (diff | hist) Week8
- 08:59, 24 July 2009 (diff | hist) N Week8 (New page: 1. Repeated FACS run of CFP and YFP with 100, 250, 500 and 1000 microM/ml IPTG 2. Preparation of requirements for transformation. 3. Kanamycin stock 100X (X= 50 microg/ml))
- 08:55, 24 July 2009 (diff | hist) N Week7 (New page: 1. Repeated FACS run of CFP and YFP with 100, 250, 500 and 1000 microM/ml IPTG)
- 08:53, 24 July 2009 (diff | hist) Week6
- 08:52, 24 July 2009 (diff | hist) N Week6 (New page: 1. FACS run of new CFP strain with different conc. of IPTG (100,250,500 microM/ml)- No signal 2. FACS run of new CFP strain with different conc. of IPTG (100,250,500,1000 microM/ml)-Sign...)
- 08:48, 24 July 2009 (diff | hist) Week5
- 08:42, 24 July 2009 (diff | hist) Team:IIT Bombay India
- 08:28, 24 July 2009 (diff | hist) N File:Logo1.gif (top)
- 08:19, 24 July 2009 (diff | hist) Team:IIT Bombay India
- 08:12, 24 July 2009 (diff | hist) N File:Iit2.jpg (top)
- 08:06, 24 July 2009 (diff | hist) N File:IitB1.jpg (top)
- 07:43, 24 July 2009 (diff | hist) N File:Iit.jpg (top)
- 07:41, 24 July 2009 (diff | hist) N File:Iit logo1.jpg (top)
- 07:26, 24 July 2009 (diff | hist) N Week5 (New page: 1.FACS run with different conc. of IPTG (100,250,500 microM/ml) for CFP and YFP-no signal(reason: sample stored after pelleting) 2.FACS run with different conc. of IPTG (100,250,500 micro...)
- 10:46, 22 July 2009 (diff | hist) Team:IIT Bombay India/Notebook
- 09:51, 22 July 2009 (diff | hist) Week1
- 09:46, 22 July 2009 (diff | hist) Team:IIT Bombay India/Notebook
- 09:36, 22 July 2009 (diff | hist) Week2
- 09:35, 22 July 2009 (diff | hist) N Week4 (New page: 1. LB growth curve of CFP, YFP and Control 2. M9 growth curve of CFP, YFP and Control)
- 09:33, 22 July 2009 (diff | hist) Week3
- 09:30, 22 July 2009 (diff | hist) Week3
- 09:27, 22 July 2009 (diff | hist) N Week2 (New page: 1.LB Amp plates 2.Antibiotic stocks Amp 1000X (X=100 microg/ml) Strep 1000X (X=500 microg/ml) 3.Plated CFP, YFP and control strains.)
- 09:19, 22 July 2009 (diff | hist) Week1
- 09:12, 22 July 2009 (diff | hist) N Week1 (New page: 1.M9,LB Preaparation 2.Ampicillin stock-100X (100 microg/ml))
- 09:07, 22 July 2009 (diff | hist) N Week3 (New page: 1.M9 media preparation 2.IPTG stock preparation 3.First FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: blank contaminated) 4.Second FACS run with 100 microM IPT...)
- 08:23, 22 July 2009 (diff | hist) Team:IIT Bombay India/Notebook
- 07:30, 22 July 2009 (diff | hist) Team:IIT Bombay India
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