1. Genomic DNA Extraction
2. Mini-Prep
3. Transformation
4. Preparation of electrocompetent E. coli
5. Yeast DNA Extraction
6. Yeast RNA Extraction
7. Purification of DNA fragments from agarose gels
8. Isolation of RNA using Trizol Reagent (Invitrogen)
9. SAP Dephosphorylation
10. CIAP Dephosphorylation
11. T4 DNA Ligase
12. Electroelution
pGEM cloning strategy
[[Team:UNICAMP-Brazil/Protocols/Preparation of electrocompetent S. cereviseae|13. Preparation of electrocompetent S. cereviseae]]
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