Team:DTU Denmark/USERprogramoutputformat

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The project


The redoxilator

- Introduction
- Results
- Applications and perspectives


The USERTM assembly standard

- Principle
- Proof of concept
- Manual


USERTM fusion primer design software

- Abstract
- Instructions
- Output format

The project


USER fusion primer design software

Program output format

Like the input options, the output from the program is kept simple. First an overview of all needed primers is shown. The output is fairly self-explanatory, although it should be noted that primer consist of a fusion tail (ft) and a binding region (br), easily distinguished from one another by the occurence of the essential uracil. For example:


|--ft--| |-------br-------|
GGGTTAAU CAAATTATAGCCATACAG


Note that the entire sequence constitute the primer, and the space is simply added for illustrative purpose, and should be omitted when synthesizing the primers

Just below is the grafic overview of biobricks and primers. The first block illustrates the forward primer for biobrick 1 Design your primers here

Synthetic Biology

“Synthetic Biology is an art of engineering new biological systems that don’t exist in nature.”

-Paras Chopra & Akhil Kamma

In nature, biological molecules work together in complex systems to serve purposes of the cell. In synthetic biology these molecules are used as individual functional units that are combined to form tailored systems exhibiting complex dynamical behaviour. From ‘design specifications’ generated from computational modelling, engineering-based approaches enables the construction of such new specified gene-regulatory networks. The ultimate goal of synthetic biology is to construct systems that gain new functions, and the perspectives of the technology are enormous. It has already been used in several medical projects2 and is predicted to play a major role in biotech-production and environmental aspects.

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