IBB Pune/8 October 2009

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I] Assembly.

Digestions:- ES Constitutive promoter Promoter RBS YcdB

XP LacO mRFP GFP RBS

BKBN A2 from fGFP

ligations Constitutive promoter + LacO Promoter RBS + mRFP Constitutive promoter + RBS YcdB + GFP Promoter RBS + GFP

II] PCR of YcdB with M13f and M13r

III] LuxI (AHL) assay with FeCl3 and hydroxylamine. FAILED :{

IV] Transformation of ligated samples