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Team:Berkeley Wetlab
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Abstract
The University of California Berkeley iGEM team aims to expand the design space of synthetic biology by exploring cell surface display. The team adopts a bottom-up design scheme in order to tackle this engineering problem in a well organized, modular fashion. In order to overcome the challenges of engineering Escherichia coli cell surface display, a high throughput, automated, combinatorial strategy is employed to generate and control the system. Using the automated system, we designed and constructed a generalizable and modular display scheme involving the autotransporter localization mechanism. We used our designed system to explore and characterize novel applications of cell surface display in Escherichia coli, the gold standard organism for bacterial engineering.
Acknowledgements
We thank our wonderful advisers: Chris Anderson, Terry Johnson, and Lane Weaver for their guidance and support. We also thank our generous sponsors:
